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Phospho-ZAK (Ser165) Antibody (PA5-105924) in ICC/IF
Immunocytochemistry analysis of Phospho-ZAK (Ser165) in A549 cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-ZAK (Ser165) polyclonal antibody (Product # PA5-105924) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(... View More
Product Details
PA5-105924
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MAP3K20(Accession Q9NYL2), corresponding to amino acid residues around phosphorylated Ser165.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817323
Product Specific Information
Antibody detects endogenous levels of ZAK only when phosphorylated at Ser165.
Target Information
ZAK is a leucine zipper-containing mitogen-activated protein related to the MLKs in both sequence and function. Like the MLKs and other MAP3Ks, ZAK activates MAP2Ks. ZAK is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif. This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HCCS-4; Human cervical cancer suppressor gene 4 protein; Leucine zipper- and sterile alpha motif kinase ZAK; Leucine zipper- and sterile alpha motif-containing kinase; Mitogen-activated protein kinase kinase kinase 20; Mitogen-activated protein kinase kinase kinase MLT; Mixed lineage kinase 7; mixed lineage kinase with a leucine zipper and a sterile alpha motif; Mixed lineage kinase-related kinase; mixed lineage kinase-related kinase MRK-beta; MLK-like mitogen-activated protein triple kinase; MLK-related kinase; MRK; sterile alpha motif and leucine zipper containing kinase AZK; Sterile alpha motif- and leucine zipper-containing kinase AZK; sterile-alpha motif and leucine zipper containing kinase AZK
Gene Aliases: AV006891; AZK; B230120H23Rik; HCCS4; MAP3K20; MLK7; mlklak; MLT; MLTK; MLTKalpha; MLTKbeta; MRK; pk; RGD1561394; SFMMP; ZAK
UniProt ID: (Human) Q9NYL2, (Mouse) Q9ESL4
Entrez Gene ID: (Human) 51776, (Mouse) 65964, (Rat) 311743
magnesium ion binding
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
poly(A) RNA binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
immunoglobulin
non-receptor serine/threonine protein kinase
cell cycle checkpoint
DNA damage checkpoint
activation of MAPKK activity
protein phosphorylation
response to stress
cytoskeleton organization
cell cycle arrest
activation of JUN kinase activity
cell death
cell proliferation
response to radiation
cell differentiation
intracellular signal transduction
embryonic digit morphogenesis
positive regulation of apoptotic process
limb development
MAPK cascade
cell cycle
phosphorylation
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