Invitrogen

Phospho-Zap-70 (Tyr493) Polyclonal Antibody

View all (97) Zap-70 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-117196

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around Y493 of human ZAP70 (NP_0010702)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.9 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901826

Product Specific Information

Positive Samples: Jurkat

Immunogen sequence: SYYTA

Target Information

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; kinase ZAP70; Selective T cell defect; syk-related protein tyrosine kinase; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; ZA70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

View more View less

Gene Aliases: ADMIO2; IMD48; mrtle; mur; SRK; STCD; STD; TZK; ZAP-70; ZAP70

View more View less

UniProt ID: (Human) P43403, (Mouse) P43404

View more View less

Entrez Gene ID: (Human) 7535, (Mouse) 22637, (Rat) 301348

View more View less

Function(s)
phosphotyrosine binding protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups molecular_function
Process(es)
adaptive immune response macrophage activation involved in immune response neutrophil activation involved in immune response protein phosphorylation inflammatory response immune response transmembrane receptor protein tyrosine kinase signaling pathway peptidyl-tyrosine phosphorylation T cell differentiation positive regulation of cell adhesion mediated by integrin intracellular signal transduction peptidyl-tyrosine autophosphorylation T cell activation B cell activation positive regulation of mast cell degranulation beta selection positive thymic T cell selection negative thymic T cell selection innate immune response positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of alpha-beta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of calcium-mediated signaling T cell receptor signaling pathway B cell receptor signaling pathway T cell aggregation T cell migration regulation of platelet aggregation immune system process phosphorylation cell migration cell differentiation thymic T cell selection protein autophosphorylation biological_process
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support