Invitrogen

Phospho-p38 MAPK Pan (Thr180, Tyr182, Thr183, Tyr185) Polyclonal Antibody

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-121361

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around T180 & Y182 of human MAPK14MAPK11 (NP_0027423)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914933

Product Specific Information

Positive test controls include: C6.

Immunogen sequence: MTGYV

Target Information

The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; ERK-6; Extracellular signal-regulated kinase 6; MAP kinase 11; MAP kinase 12; MAP kinase 13; MAP kinase 14; MAP kinase 2; MAP kinase MXI2; MAP kinase p38 alpha; MAP kinase p38 beta; MAP kinase p38 delta; MAP kinase p38 gamma; MAPK 12; MAPK 13; MAPK 14; MAX-interacting protein 2; Mitogen-activated protein kinase 11; Mitogen-activated protein kinase 12; Mitogen-activated protein kinase 13; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 alpha; Mitogen-activated protein kinase p38 beta; Mitogen-activated protein kinase p38 delta; Mitogen-activated protein kinase p38 gamma; mitogen-activated protein kinase p38-2; p38 MAP kinase; p38 mitogen activated protein kinase; p38-2; p38alpha Exip; reactive kinase; SAP kinase-3; SAPK2a; SAPK2b; Stress-activated protein kinase 2a; Stress-activated protein kinase 2b; Stress-activated protein kinase 3; Stress-activated protein kinase 4; stress-activated protein kinase-2; stress-activated protein kinase-2b

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Gene Aliases: CRK1; CSBP; CSBP1; CSBP2; CSPB1; ERK-6; ERK3; ERK6; EXIP; Hog; MAPK 12; MAPK 13; MAPK-13; MAPK11; MAPK12; MAPK13; MAPK14; MXI2; p38; p38-2; p38ALPHA; P38B; p38Beta; P38BETA2; p38delta; P38GAMMA; p38Hog; PRKM11; PRKM12; PRKM13; PRKM14; PRKM15; RK; SAPK-3; SAPK2; SAPK2A; SAPK2B; SAPK3; SAPK4

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UniProt ID: (Human) Q15759, (Human) P53778, (Human) O15264, (Human) Q16539, (Rat) Q63538

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Entrez Gene ID: (Human) 5600, (Human) 6300, (Human) 5603, (Human) 1432, (Rat) 689314, (Rat) 60352, (Rat) 29513, (Rat) 81649

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Function(s)
protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding magnesium ion binding MAP kinase kinase activity protein C-terminus binding enzyme binding protein phosphatase binding NFAT protein binding protein kinase activity kinase activity non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade activation of MAPK activity transcription, DNA-templated response to stress signal transduction Ras protein signal transduction positive regulation of gene expression intracellular signal transduction positive regulation of erythrocyte differentiation vascular endothelial growth factor receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity positive regulation of muscle cell differentiation negative regulation of cardiac muscle cell proliferation cellular response to virus regulation of signal transduction by p53 class mediator positive regulation of interleukin-12 secretion regulation of transcription, DNA-templated DNA damage induced protein phosphorylation cell cycle arrest muscle organ development positive regulation of peptidase activity peptidyl-serine phosphorylation myoblast differentiation response to osmotic stress cell cycle positive regulation of interleukin-6 production positive regulation of inflammatory response DNA damage checkpoint cell morphogenesis cartilage condensation angiogenesis placenta development chondrocyte differentiation glucose metabolic process regulation of transcription from RNA polymerase II promoter apoptotic process movement of cell or subcellular component chemotaxis cell surface receptor signaling pathway transmembrane receptor protein serine/threonine kinase signaling pathway skeletal muscle tissue development response to glucose positive regulation of myotube differentiation myoblast differentiation involved in skeletal muscle regeneration fatty acid oxidation osteoclast differentiation positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway response to muramyl dipeptide cellular response to vascular endothelial growth factor stimulus response to muscle stretch p38MAPK cascade positive regulation of protein import into nucleus signal transduction in response to DNA damage positive regulation of blood vessel endothelial cell migration positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import protein autophosphorylation striated muscle cell differentiation positive regulation of cardiac muscle cell proliferation 3'-UTR-mediated mRNA stabilization cellular response to lipopolysaccharide cellular response to ionizing radiation negative regulation of canonical Wnt signaling pathway positive regulation of brown fat cell differentiation stress-induced premature senescence regulation of cytokine production involved in inflammatory response positive regulation of myoblast fusion positive regulation of reactive oxygen species metabolic process protein phosphorylation regulation of cardiac muscle cell proliferation negative regulation of cell cycle cellular response to DNA damage stimulus response to lipopolysaccharide stress-activated MAPK cascade
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