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Prolactin Receptor (ECD) Antibody (PA5-112169) in WB
Western Blot analysis of ECD using Prolactin Receptor (ECD) Polyclonal Antibody (Product # PA5-112169) at a dilution of 1:500. Lane A: Jurkat whole cell lysate, Lane B: HeLa whole cell lysate, Lane C: 293T whole cell lysate, Lane D: U-251MG whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL t... View More
Product Details
PA5-112169
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived Human ECD fragment
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2866905
Target Information
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ecdysoneless homolog; hSGT1; Human suppressor of GCR two; Protein ecdysoneless homolog; protein SGT1; SGT1 protein; suppressor of GCR2; suppressor of S. cerevisiae gcr2
Gene Aliases: ECD; GCR2; HSGT1; SGT1
UniProt ID: (Human) O95905
Entrez Gene ID: (Human) 11319
Performance Guarantee
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