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FIGURE: 1 / 3
QKI Antibody (MA5-45690) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with QKI (pan) monoclonal antibody (Product # MA5-45690) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-... View More
Product Details
MA5-45690
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
N147/6
Immunogen
Fusion protein amino acids 1-341 (full-length) of human QKI-5.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932144
Product Specific Information
Mouse: 100% identity (341/341 amino acids identical). Rat: 99% identity (339/341 amino acids identical) >90% identity with QKI-6, QKI-7 and QKI-7b.
1 µg/mL of MA5-45690 was sufficient for detection of Pan-QKI in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 36-38kDa.
This antibody was formerly sold as clone S147-6.
Target Information
This gene encodes the sacsin protein, which includes a UbL domain at the N-terminus, a DnaJ domain, and a HEPN domain at the C-terminus. The gene is highly expressed in the central nervous system, also found in skin, skeletal muscles and at low levels in the pancreas. This gene includes a very large exon spanning more than 12.8 kb. Mutations in this gene result in autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), a neurodegenerative disorder characterized by early-onset cerebellar ataxia with spasticity and peripheral neuropathy. Sacsin has been found to protect against mutant ataxin-1. A pseudogene associated with this gene is located on chromosome 11. Alternative splicing of this gene results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp586I0923; homolog of mouse quaking QKI (KH domain RNA binding protein); Hqk; KH domain-containing RNA-binding protein QKI; lethal, Chr 17, U Wisconsin 1; MqkI; Protein quaking; protein quaking-like; QKI, KH domain containing, RNA binding; QKI-5 protein; quaking homolog, KH domain RNA binding; RNA Binding (QKI); RNA binding protein HQK; rqkI
Gene Aliases: 1110003F05Rik; 1500005P18; HKQ; Hqk; hqkI; l(17)-1Wis; l17Wis1; QK; QK1; QK3; QKI
UniProt ID: (Human) Q96PU8, (Mouse) Q9QYS9
Entrez Gene ID: (Human) 9444, (Mouse) 19317, (Rat) 499022, (Rat) 100910212
vasculogenesis
mRNA processing
spermatid development
RNA splicing
positive regulation of gene expression
myelination
muscle cell differentiation
long-chain fatty acid biosynthetic process
mRNA transport
3'-UTR-mediated mRNA destabilization
regulation of translation
transport
multicellular organism development
axon ensheathment
cell differentiation
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