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RAB1A Antibody (PA5-27421) in ICC/IF
Knockout of RAF1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR627776_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of RAF1 was performed by loading 30 µg of HeLa wild type (Lane 1), HeLa Cas9 (Lane 2) and HeLa RAF1 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by i... View More
Product Details
PA5-27421
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 205 of Human Rab 1A
Conjugate
Form
Liquid
Concentration
0.26 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2544897
Product Specific Information
Recommended positive controls: U87-MG, SK-N-SH, rat brain, Rab 1A tansfected 293T cell.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (98%), Dog (100%), Pig (99%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (80%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). This gene is co-transcribed with the U68 snoRNA, located within the third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZP564B163; GTP binding protein Rab1a; Rab GTPase YPT1 homolog; RAB1, member RAS oncogene family; Ras-related protein Rab-1A; ras-related YPT1 protein; YPT1-related protein
Gene Aliases: Ac2-048; Gtbp; mKIAA3012; Rab-1; RAB1; RAB1A; Rab1r; YPT1
UniProt ID: (Human) P62820, (Mouse) P62821, (Rat) Q6NYB7
Entrez Gene ID: (Human) 5861, (Mouse) 19324, (Rat) 81754
autophagosome assembly
intracellular protein transport
ER to Golgi vesicle-mediated transport
endocytosis
autophagy
cell migration
virion assembly
growth hormone secretion
Rab protein signal transduction
defense response to bacterium
vesicle transport along microtubule
interleukin-8 secretion
establishment of endothelial intestinal barrier
positive regulation of glycoprotein metabolic process
regulation of autophagosome assembly
retrograde vesicle-mediated transport, Golgi to ER
Golgi organization
small GTPase mediated signal transduction
vesicle-mediated transport
melanosome transport
substrate adhesion-dependent cell spreading
cilium assembly
COPII vesicle coating
cargo loading into COPII-coated vesicle
cell-cell adhesion
transport
protein transport
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