Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
RNF216 Antibody (PA1-41317) in WB
Western blot analysis of RNF216 in human (A) Brain; (B) Heart; (C) Small Intestine; (D) Kidney; (E) Liver; (F) Lung; (G) Skeletal Muscle; (H) Pancreas; (I) Spleen; (J) Ovary; (K) Testis. Samples were incubated in RNF216 polyclonal antibody (Product # PA1-41317) using a dilution of 1 µg/mL.
Product Details
PA1-41317
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues 350-400 of human TRIAD3A.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS with 0.2% gelatin
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2180807
Product Specific Information
Suggested positive control: human spleen protein, antigen standard for RNF216 (transient overexpression lysate), human lung protein, human ovary protein, human pancreas protein, human skeletal muscle protein, human heart protein, human kidney protein, human liver protein, human brain protein, human brain tissue, heart tissue, kidney tissue, liver tissue, lung tissue, ovary tissue, pancreas tissue, small intestine tissue, skeletal muscle tissue, spleen tissue, human small intestine protein.
Target Information
Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RNF216; RING finger protein 216; RING-type E3 ubiquitin transferase RNF216; Triad domain-containing protein 3; triad3a; Ubiquitin-conjugating enzyme 7-interacting protein 1; Zinc finger protein inhibiting NF-kappa-B
Gene Aliases: CAHH; RNF216; TRIAD3; U7I1; UBCE7IP1; ZIN
UniProt ID: (Human) Q9NWF9
Entrez Gene ID: (Human) 54476
apoptotic process
viral process
negative regulation of type I interferon production
regulation of interferon-beta production
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of defense response to virus by host
protein K48-linked ubiquitination
protein linear polyubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support