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Invitrogen
RNF216 Polyclonal Antibody
Product Details
PA5-20079
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11155204
Product Specific Information
A suggested positive control is mouse heart tissue lysate.
PA5-20079 can be used with blocking peptide PEP-0197.
Target Information
Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E3 ubiquitin ligase; E3 ubiquitin-protein ligase RNF216; RING finger protein 216; RING-type E3 ubiquitin transferase RNF216; Triad domain-containing protein 3; triad3a; UbcM4-interacting protein 83; Ubiquitin-conjugating enzyme 7-interacting protein 1; Zinc finger protein inhibiting NF-kappa-B
Gene Aliases: 2810055G22Rik; AI647468; AU019462; C86502; CAHH; F830018F18Rik; RNF216; TRIAD3; U7I1; UBCE7IP1; UIP83; ZIN
UniProt ID: (Human) Q9NWF9, (Mouse) P58283
Entrez Gene ID: (Human) 54476, (Mouse) 108086
ubiquitin-protein transferase activity
protein binding
ligase activity
ubiquitin binding
metal ion binding
ubiquitin protein ligase activity
molecular_function
zinc ion binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
apoptotic process
viral process
negative regulation of type I interferon production
regulation of interferon-beta production
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of defense response to virus by host
protein K48-linked ubiquitination
protein linear polyubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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