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RSK1 Antibody (MA5-35559) in WB
Western blot analysis of RSK1 in extracts of various cell lines. Samples were incubated with RSK1 Monoclonal antibody (Product # MA5-35559) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
MA5-35559
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC1096
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p90Rsk/RSK1/RPS6KA1 (Q15418)
Conjugate
Form
Liquid
Concentration
1.4 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849459
Product Specific Information
Immunogen sequence: MPLAQLKEPW PLMELVPLDP ENGQTSGEEA GLQPSKDEGV LKEISITHHV KAGSEKADPS HFELLKVLGQ GSFGKVFLVR KVTRPDSGHL YAMKVLKKAT
Target Information
RPS6KA1 (RSK1) is a growth-factor regulated serine/threonine kinase that is involved in the MAPK cascade. It is thought to control cellular proliferation and differentiation through the phosphorylation of transcription factors. RPS6KA1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; p90-RSK 1; p90RSK1; p90S6K; ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RSK-1; S6K-alpha 1; S6K-alpha-1
Gene Aliases: HU-1; Mapkapk-1a; MAPKAPK1A; p90-Rsk1; p90Rsk; p90Rsk1; p90S6K; RPS6KA1; RSK; Rsk-1; RSK1; S6K-alpha-1
UniProt ID: (Human) Q15418, (Mouse) P18653
Entrez Gene ID: (Human) 6195, (Mouse) 20111
magnesium ion binding
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
kinase activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
nucleotide binding
protein kinase activity
ribosomal protein S6 kinase activity
transcription factor binding
transferase activity
transferase activity, transferring phosphorus-containing groups
protein modifying enzyme
protein phosphorylation
apoptotic process
cell cycle
signal transduction
positive regulation of cell growth
intracellular signal transduction
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of translation in response to stress
regulation of DNA-templated transcription in response to stress
positive regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
hepatocyte proliferation
positive regulation of hepatic stellate cell activation
regulation of translation
phosphorylation
positive regulation of transcription, DNA-templated
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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