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RTRAF Antibody (PA5-92009) in WB
Western blot analysis of RTRAF in extracts of various cell lines. Samples were incubated with RTRAF Polyclonal antibody (Product # PA5-92009) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-92009
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human C14orf166 (NP_0571231)
Conjugate
Form
Liquid
Concentration
1.02 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806351
Product Specific Information
Immunogen sequence: MFRRKLTALD YHNPAGFNCK DETEFRNFIV WLEDQKIRHY KIEDRGNLRN IHSSDWPKFF EKYLRDVNCP FKIQDRQEAI DWLLGLAVRL EYGDNAEKYK DLVPDNSKTA DNATKNAEPL INLDVNNPDF KAGVMALANL LQIQRHDDYL VMLKAIRILV QERLTQDAVA KANQTKEGLP VALDKHILGF DTGDAVLNEA AQILRLLHIE ELRELQTKIN EAIVAVQAII ADPKTDHRLG KVGR; Positive Samples: Jurkat, 293T; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, cytosol, microtubule organizing center, perinuclear region
Target Information
RTRAF (C14orf166) is an RNA-binding protein involved in modulation of mRNA transcription by Polymerase II (PubMed:16950395). Component of the tRNA-splicing ligase complex and is required for tRNA ligation (PubMed:24870230). May be required for RNA transport (PubMed:24608264).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CLE; CLE7 homolog; RLL motif containing 1; RNA transcription, translation and transport factor protein; UPF0568 protein C14orf166 homolog
Gene Aliases: C14orf166; CGI-99; CGI99; CLE; CLE7; hCLE1; LCRP369; RLLM1; RTRAF
UniProt ID: (Mouse) Q9CQE8, (Human) Q9Y224
Entrez Gene ID: (Mouse) 68045, (Human) 51637
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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