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Rhodopsin Antibody (MA5-45247) in IHC (P)
Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Rhodopsin monoclonal antibody (Product # MA5-45247) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.
Product Details
MA5-45247
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1D4
Immunogen
Bovine Rhodopsin
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931701
Product Specific Information
1 µg/mL of MA5-45247 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 40kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
Target Information
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; OPN 2; opsin 2; opsin 2, rod pigment; Opsin-2; Red Opsin; Rhodopsin; Rod Opsin; RP 4
Gene Aliases: CSNBAD1; Noerg1; OPN2; Ops; RHO; RP4
UniProt ID: (Human) P08100, (Mouse) P15409
Entrez Gene ID: (Human) 6010, (Mouse) 212541
retinoid metabolic process
protein phosphorylation
G-protein coupled receptor signaling pathway
visual perception
phototransduction, visible light
red, far-red light phototransduction
absorption of visible light
rhodopsin mediated signaling pathway
protein-chromophore linkage
regulation of rhodopsin mediated signaling pathway
photoreceptor cell maintenance
retina development in camera-type eye
signal transduction
phototransduction
response to light stimulus
detection of light stimulus
response to stimulus
sensory perception of light stimulus
cellular response to light stimulus
Performance Guarantee
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