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Rhodopsin Antibody (MA5-45245) in IHC
Immunohistochemistry analysis using retina. Samples were incubated with Rhodopsin monoclonal antibody (Product # MA5-45245) at 1:1,000, followed by FITC Goat Anti-Mouse (green). Counterstain used was DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS – inner segment; ONL – outer nuclear layer; OPL – outer plexiform layer; INL – inner nuclear layer; IPL – inner plexiform layer; GCL – ganglion cell layer.
Product Details
MA5-45245
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Amphibian,
Avian,
Bovine,
Fish,
Human,
Mouse,
Shark
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4D2
Immunogen
Bovine Rhodopsin
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.09mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931699
Product Specific Information
1 µg/mL of MA5-45245 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
Target Information
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; OPN 2; opsin 2; opsin 2, rod pigment; Opsin-2; Red Opsin; Rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); Rod Opsin; RP 4
Gene Aliases: CSNBAD1; Noerg1; OPN2; Ops; RHO; RP4
UniProt ID: (Bovine) P02699, (Human) P08100, (Mouse) P15409
Entrez Gene ID: (Bovine) 509933, (Human) 6010, (Mouse) 212541
G-protein alpha-subunit binding
opsin binding
guanyl-nucleotide exchange factor activity
11-cis retinal binding
protein binding
G-protein coupled photoreceptor activity
zinc ion binding
identical protein binding
arrestin family protein binding
G-protein coupled receptor activity
retinal binding
metal ion binding
signal transducer activity
photoreceptor activity
spectrin binding
G-protein coupled receptor
protein phosphorylation
visual perception
phototransduction, visible light
response to light stimulus
absorption of visible light
rhodopsin mediated signaling pathway
protein-chromophore linkage
photoreceptor cell maintenance
retina development in camera-type eye
retinoid metabolic process
G-protein coupled receptor signaling pathway
red, far-red light phototransduction
regulation of rhodopsin mediated signaling pathway
signal transduction
phototransduction
detection of light stimulus
response to stimulus
sensory perception of light stimulus
cellular response to light stimulus
Performance Guarantee
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