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Product Details
PA5-38017
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
16 amino acid peptide near the amino terminus of human Rubicon
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2554621
Product Specific Information
A recommended positive control is 293 cell lysate.
Multiple isoforms of Rubicon are known to exist.
Target Information
Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation. Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex. Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Baron; Beclin-1 associated RUN domain containing protein; Rubicon; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; run domain Beclin-1 interacting and cystein-rich containing protein; run domain Beclin-1 interacting and cysteine-rich containing protein; Run domain Beclin-1-interacting and cysteine-rich domain-containing protein; RUN domain protein as Beclin 1-interacting and cysteine-rich containing; rundataxin; similar to mKIAA0226 protein
Gene Aliases: 5330403K09; KIAA0226; mKIAA0226; RUBCN; RUBICON; SCAR15
UniProt ID: (Mouse) Q80U62, (Human) Q92622
Entrez Gene ID: (Mouse) 100502698, (Rat) 303885, (Human) 9711
biological_process
negative regulation of autophagy
negative regulation of endocytosis
immune system process
phagocytosis
negative regulation of phosphatidylinositol 3-kinase activity
multivesicular body sorting pathway
autophagosome maturation
negative regulation of autophagosome maturation
endocytosis
autophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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