Invitrogen

SAP97 Polyclonal Antibody

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View all (16) SAP97 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA1-044

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues E(16) E Y R S K L S Q T E D R Q L R S S(33) of human SAP97.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2092021

Product Specific Information

PA1-044 detects synapse-associated protein 97 (SAP97) from rat and human tissues. This antibody does not detect other synapse-associated protein family members.

PA1-044 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~140 kDa protein representing SAP97 from rat brain homogenate.

The PA1-044 immunogen is a synthetic peptide corresponding to residues E(16) E Y R S K L S Q T E D R Q L R S S(33) of human SAP97. This sequence is completely conserved in rat and mouse. PA1-044 immunizing peptide (Cat. # PEP-022) is available for use in neutralization and control experiments.

Target Information

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: discs large homolog 1; discs large homolog 1, scribble cell polarity complex component; discs, large homolog 1; discs, large homolog 1 (Drosophila) transcript variant 6-v1; discs, large homolog 1 (Drosophila) transcript variant 6-v2; Disks large homolog 1; DKFZp761P0818; DKFZp781B0426; Drosophila discs-large tumor suppressor homologue (synapse associated protein); hDlg; presynaptic protein SAP97; SAP-97; Synapse-associated protein 97

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Gene Aliases: dJ1061C18.1.1; DLG1; DLGH1; hdlg; SAP-97; SAP97

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UniProt ID: (Human) Q12959, (Rat) Q62696

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Entrez Gene ID: (Human) 1739, (Rat) 25252

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Function(s)
guanylate kinase activity phosphoprotein phosphatase activity protein binding protein C-terminus binding cytoskeletal protein binding ligand-gated ion channel activity potassium channel regulator activity protein kinase binding phosphatase binding mitogen-activated protein kinase kinase binding protein complex scaffold ionotropic glutamate receptor binding ion channel binding L27 domain binding cadherin binding involved in cell-cell adhesion PDZ domain binding scaffold/adaptor protein
Process(es)
negative regulation of transcription from RNA polymerase II promoter branching involved in ureteric bud morphogenesis immunological synapse formation endothelial cell proliferation lens development in camera-type eye T cell cytokine production protein dephosphorylation actin filament organization mitotic cell cycle checkpoint establishment or maintenance of cell polarity chemical synaptic transmission nervous system development positive regulation of cell proliferation regulation of cell shape viral process single organismal cell-cell adhesion peristalsis positive regulation of actin filament polymerization cortical actin cytoskeleton organization astral microtubule organization membrane raft organization regulation of myelination activation of protein kinase activity ion transmembrane transport cellular protein complex localization T cell activation negative regulation of T cell proliferation regulation of membrane potential amyloid precursor protein metabolic process receptor clustering positive regulation of potassium ion transport cortical microtubule organization establishment or maintenance of epithelial cell apical/basal polarity negative regulation of mitotic cell cycle GMP metabolic process GDP metabolic process reproductive structure development embryonic skeletal system morphogenesis smooth muscle tissue development negative regulation of epithelial cell proliferation establishment of centrosome localization negative regulation of protein kinase B signaling hard palate development negative regulation of ERK1 and ERK2 cascade bicellular tight junction assembly protein localization to plasma membrane positive regulation of establishment of protein localization to plasma membrane receptor localization to synapse regulation of NIK/NF-kappaB signaling regulation of sodium ion transmembrane transport negative regulation of p38MAPK cascade ureteric bud development cell adhesion protein localization regulation of protein localization nucleotide phosphorylation tissue morphogenesis
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