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Invitrogen
SDHA Recombinant Rabbit Monoclonal Antibody (JM10-83)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 15
SDHA Antibody (MA5-32598) in ICC/IF
Immunofluorescence analysis of SDHA was performed using 70 confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SDHA Recombinant Rabbit Monoclonal Antibody (JM10-83) (Product # MA5-32598) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor ... View More
Product Details
MA5-32598
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JM10-83
Immunogen
Synthetic peptide within Human SDHA aa 581-624
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809875
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Flavoprotein subunit of complex II; Fp; Malate dehydrogenase [quinone] flavoprotein subunit; succinate dehydrogenase [ubiquinone] flavoprotein subunit; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; succinate dehydrogenase complex subunit A, flavoprotein (Fp); succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Gene Aliases: 1500032O14Rik; 2310034D06Rik; 4921513A11; C81073; CMD1GG; FP; im:7141001; PGL5; SDH1; SDH2; SDHA; SDHF; si:dkeyp-84f11.2; zgc:56051
UniProt ID: (Human) Q59GW8, (Rat) Q920L2, (Mouse) Q8K2B3, (Zebrafish) Q7ZVF3
Entrez Gene ID: (Human) 6389, (Rat) 157074, (Mouse) 66945, (Zebrafish) 393884
succinate dehydrogenase activity
protein binding
succinate dehydrogenase (ubiquinone) activity
flavin adenine dinucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on the CH-CH group of donors
electron carrier activity
dehydrogenase
oxidoreductase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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