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Product Details
H00006464-M01
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
3F4
Immunogen
SHC1 (AAH33925, 171 a.a. approximately 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Product may be used with Western Blot (Cell lysate).
Immunogen sequence: QTPSHLGATL PVGQPVGGDP EVRKQMPPPP PCPGRELFDD PSYVNVQNLD KARQAVGGAG PPNPAINGSA PRDLFDMKPF EDALRVPPPP QSVSMAEQLR GEPWFHGKLS
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ26504; OTTHUMP00000035472; RP11-307C12.1; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC (Src homology 2 domain-containing) transforming protein 1; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1
Gene Aliases: SHC; SHC1; SHCA
UniProt ID: (Human) P29353
Entrez Gene ID: (Human) 6464
phosphotyrosine binding
transmembrane receptor protein tyrosine kinase adaptor activity
Ras guanyl-nucleotide exchange factor activity
epidermal growth factor receptor binding
insulin receptor binding
insulin-like growth factor receptor binding
neurotrophin TRKA receptor binding
protein binding
phospholipid binding
ephrin receptor binding
protein phosphatase 2A binding
scaffold/adaptor protein
MAPK cascade
activation of MAPK activity
angiogenesis
response to hypoxia
epidermal growth factor receptor signaling pathway
regulation of epidermal growth factor-activated receptor activity
Ras protein signal transduction
heart development
aging
positive regulation of cell proliferation
insulin receptor signaling pathway
response to toxic substance
viral process
single organismal cell-cell adhesion
organ regeneration
neuron projection development
actin cytoskeleton reorganization
response to nicotine
IRE1-mediated unfolded protein response
Fc-epsilon receptor signaling pathway
ERBB2 signaling pathway
regulation of growth
response to hydrogen peroxide
positive regulation of GTPase activity
positive regulation of DNA replication
positive regulation of vasoconstriction
positive regulation of smooth muscle cell proliferation
leukocyte migration
response to glucocorticoid
cellular response to growth factor stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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