Proteintech

SHP2 Polyclonal Antibody

SHP2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 22

SHP2 Antibody (20145-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using PTPN11 antibody 20145-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

SHP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SHP2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20145-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SHP2 Fusion Protein Ag13660 (1-357 aa encoded by BC008692)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTSRRWFHP NITGVEAENL LLTRGVDGSF LARPSKSNPG DFTLSVRRNG AVTHIKIQNT GDYYDLYGGE KFATLAELVQ YYMEHHGQLK EKNGDVIELK YPLNCADPTS ERWFHGHLSG KEAEKLLTEK GKHGSFLVRE SQSHPGDFVL SVRTGDDKGE SNDGKSKVTH VMIRCQELKY DVGGGERFDS LTDLVEHYKK NPMVETLGTV LQLKQPLNTT RINAAEIESR VRELSKLAET TDKVKQGFWE EFETLQQQEC KLLYSRKEGQ RQENKNKNRY KNILPFDHTR VVLHDGDPNE PVSDYINANI IMPEFETKCN NSKPKKSYIA TQGCLQNTVN DFWRMVFQEN SRVIVMTT (1-357 aa encoded by BC008692)

Target Information

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Mon May 29 00:00:00 EDT 2023

Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance.

Cell reports

Gao X,Wu Y,Chick JM,Abbott A,Jiang B,Wang DJ,Comte-Walters S,Johnson RH,Oberholtzer N,Nishimura MI,Gygi SP,Mehta A,Guttridge DC,Ball L,Mehrotra S,Sicinski P,Yu XZ,Wang H

Tue Apr 25 00:00:00 EDT 2023

Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance.

Cell reports

Gao X,Wu Y,Chick JM,Abbott A,Jiang B,Wang DJ,Comte-Walters S,Johnson RH,Oberholtzer N,Nishimura MI,Gygi SP,Mehta A,Guttridge DC,Ball L,Mehrotra S,Sicinski P,Yu XZ,Wang H

Tue Apr 25 00:00:00 EDT 2023

Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways.

Toxicology and applied pharmacology

Fu NJ,Xi RY,Shi XK,Li RZ,Zhang ZH,Li LY,Zhang GL,Wang F

Fri Apr 15 00:00:00 EDT 2022

Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways.

Toxicology and applied pharmacology

Fu NJ,Xi RY,Shi XK,Li RZ,Zhang ZH,Li LY,Zhang GL,Wang F

Fri Apr 15 00:00:00 EDT 2022

Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells.

Cell chemical biology

Zhang R,Chen D,Fan H,Wu R,Tu J,Zhang FQ,Wang M,Zheng H,Qu CK,Elf SE,Faubert B,He YY,Bissonnette MB,Gao X,DeBerardinis RJ,Chen J

Thu Jul 21 00:00:00 EDT 2022

A novel 2B4 receptor leads to worse pregnancy outcomes by facilitating TNF-α and IFN-γ production in dNK cells during Toxoplasma gondii infection.

Parasites & vectors

Xu X,Zheng G,Ren Y,He X,Peng B,Hu X,Liu W

Sat Sep 24 00:00:00 EDT 2022

A novel 2B4 receptor leads to worse pregnancy outcomes by facilitating TNF-α and IFN-γ production in dNK cells during Toxoplasma gondii infection.

Parasites & vectors

Xu X,Zheng G,Ren Y,He X,Peng B,Hu X,Liu W

Sat Sep 24 00:00:00 EDT 2022

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Analytical chemistry

Chu H,Zhao Q,Liu J,Yang K,Wang Y,Liu J,Zhang K,Zhao B,He H,Zheng Y,Zhong S,Liang Z,Zhang L,Zhang Y

Tue Jan 18 00:00:00 EST 2022

miR-6071 inhibits hepatocellular carcinoma progression via targeting PTPN11.

Archives of biochemistry and biophysics

Chen M,Wang H,Shi S,Zhang H,Xu S,Jiang Y

Fri Sep 30 00:00:00 EDT 2022

Structure-based discovery of a specific SHP2 inhibitor with enhanced blood-brain barrier penetration from PubChem database.

Bioorganic chemistry

Ma Y,Li WY,Sun T,Zhang L,Lu XH,Yang B,Wang RL

Fri Apr 01 00:00:00 EDT 2022

Inhibition of Phosphodiesterase 5 Promotes the Aromatase-Mediated Estrogen Biosynthesis in Osteoblastic Cells by Activation of cGMP/PKG/SHP2 Pathway.

Frontiers in endocrinology

Wisanwattana W,Wongkrajang K,Cao DY,Shi XK,Zhang ZH,Zhou ZY,Li F,Mei QG,Wang C,Suksamrarn A,Zhang GL,Wang F

Tue Dec 21 00:00:00 EST 2021

Shp2 activation in bone marrow microenvironment mediates the drug resistance of B-cell acute lymphoblastic leukemia through enhancing the role of VCAM-1/VLA-4.

International immunopharmacology

Yu K,Yin Y,Ma D,Lu T,Wei D,Xiong J,Zhou Z,Zhang T,Zhang S,Fang Q,Wang J

Sun Mar 01 00:00:00 EST 2020

Shp2 activation in bone marrow microenvironment mediates the drug resistance of B-cell acute lymphoblastic leukemia through enhancing the role of VCAM-1/VLA-4.

International immunopharmacology

Yu K,Yin Y,Ma D,Lu T,Wei D,Xiong J,Zhou Z,Zhang T,Zhang S,Fang Q,Wang J

Sun Mar 01 00:00:00 EST 2020

The role of tyrosine phosphatase Shp2 in spermatogonial differentiation and spermatocyte meiosis.

Asian journal of andrology

Li Y,Liu WS,Yi J,Kong SB,Ding JC,Zhao YN,Tian YP,Feng GS,Li CJ,Liu W,Wang HB,Lu ZX

Mon Nov 30 00:00:00 EST 2020

Brevilin A Inhibits STAT3 Signaling and Induces ROS-Dependent Apoptosis, Mitochondrial Stress and Endoplasmic Reticulum Stress in MCF-7 Breast Cancer Cells.

OncoTargets and therapy

Saleem MZ,Nisar MA,Alshwmi M,Din SRU,Gamallat Y,Khan M,Ma T

Tue Apr 12 00:00:00 EDT 2022

Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.

Aging

Shi C,Yang J,Hu L,Liao B,Qiao L,Shen W,Xie F,Zhu G

Mon Aug 03 00:00:00 EDT 2020

miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance.

Molecular therapy. Nucleic acids

Han T,Zhang Y,Yang X,Han L,Li H,Chen T,Zheng Z

Fri Mar 06 00:00:00 EST 2020

Anticancer activity of dietary xanthone α-mangostin against hepatocellular carcinoma by inhibition of STAT3 signaling via stabilization of SHP1.

Cell death & disease

Zhang H,Tan YP,Zhao L,Wang L,Fu NJ,Zheng SP,Shen XF

Fri Jan 24 00:00:00 EST 2020

Inhibition of JNK-Mediated Autophagy Promotes Proscillaridin A- Induced Apoptosis via ROS Generation, Intracellular Ca⁺² Oscillation and Inhibiting STAT3 Signaling in Breast Cancer Cells.

Frontiers in pharmacology

Saleem MZ,Alshwmi M,Zhang H,Din SRU,Nisar MA,Khan M,Alam S,Alam G,Jin L,Ma T

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Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.

Oncogene

Patel Y,Soni M,Awgulewitsch A,Kern MJ,Liu S,Shah N,Singh UP,Chen H

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Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.

Oncogene

Patel Y,Soni M,Awgulewitsch A,Kern MJ,Liu S,Shah N,Singh UP,Chen H

Tue Jan 01 00:00:00 EST 2019

A thiazole-derived oridonin analogue exhibits antitumor activity by directly and allosterically inhibiting STAT3.

The Journal of biological chemistry

Shen X,Zhao L,Chen P,Gong Y,Liu D,Zhang X,Dai L,Sun Q,Lou J,Jin Z,Zhang B,Niu D,Chen C,Qi X,Jia D

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Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.

Oxidative medicine and cellular longevity

Maryam A,Mehmood T,Yan Q,Li Y,Khan M,Ma T

Tue Sep 18 00:00:00 EDT 2018

Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.

Oxidative medicine and cellular longevity

Maryam A,Mehmood T,Yan Q,Li Y,Khan M,Ma T

Tue Sep 18 00:00:00 EDT 2018

Chemical and genetic inhibition of STAT3 sensitizes hepatocellular carcinoma cells to sorafenib induced cell death.

International journal of biological sciences

Xie L,Zeng Y,Dai Z,He W,Ke H,Lin Q,Chen Y,Bu J,Lin D,Zheng M

Mon Jul 15 00:00:00 EDT 2019

Chemical and genetic inhibition of STAT3 sensitizes hepatocellular carcinoma cells to sorafenib induced cell death.

International journal of biological sciences

Xie L,Zeng Y,Dai Z,He W,Ke H,Lin Q,Chen Y,Bu J,Lin D,Zheng M

Mon Jul 15 00:00:00 EDT 2019

Proscillaridin A induces apoptosis, inhibits STAT3 activation and augments doxorubicin toxicity in prostate cancer cells.

International journal of medical sciences

He Y,Khan M,Yang J,Yao M,Yu S,Gao H

Mon Dec 17 00:00:00 EST 2018

Proscillaridin A induces apoptosis, inhibits STAT3 activation and augments doxorubicin toxicity in prostate cancer cells.

International journal of medical sciences

He Y,Khan M,Yang J,Yao M,Yu S,Gao H

Mon Dec 17 00:00:00 EST 2018

The abnormal activation of D1R/Shp-2 complex involved in levodopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson's rats.

Neuropsychiatric disease and treatment

Wu N,Wan Y,Song L,Qi C,Liu Z,Gan J

Fri Mar 18 00:00:00 EDT 2022

The abnormal activation of D1R/Shp-2 complex involved in levodopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson's rats.

Neuropsychiatric disease and treatment

Wu N,Wan Y,Song L,Qi C,Liu Z,Gan J

Fri Mar 18 00:00:00 EDT 2022

Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.

Hepatology (Baltimore, Md.)

Xiang D,Cheng Z,Liu H,Wang X,Han T,Sun W,Li X,Yang W,Chen C,Xia M,Liu N,Yin S,Jin G,Lee T,Dong L,Hu H,Wang H,Ding J

Mon May 01 00:00:00 EDT 2017

Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.

Hepatology (Baltimore, Md.)

Xiang D,Cheng Z,Liu H,Wang X,Han T,Sun W,Li X,Yang W,Chen C,Xia M,Liu N,Yin S,Jin G,Lee T,Dong L,Hu H,Wang H,Ding J

Mon May 01 00:00:00 EDT 2017

Levodopa/Benserazide Loaded Microspheres Alleviate L-dopa Induced Dyskinesia through Preventing the Over-Expression of D1R/Shp-2/ERK1/2 Signaling Pathway in a Rat Model of Parkinson's Disease.

Frontiers in aging neuroscience

Wan Y,Wu N,Song L,Wang X,Liu Z,Yuan W,Gan J

Wed Sep 30 00:00:00 EDT 2020

Levodopa/Benserazide Loaded Microspheres Alleviate L-dopa Induced Dyskinesia through Preventing the Over-Expression of D1R/Shp-2/ERK1/2 Signaling Pathway in a Rat Model of Parkinson's Disease.

Frontiers in aging neuroscience

Wan Y,Wu N,Song L,Wang X,Liu Z,Yuan W,Gan J

Wed Sep 30 00:00:00 EDT 2020

Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Oncotarget

Igbe I,Shen XF,Jiao W,Qiang Z,Deng T,Li S,Liu WL,Liu HW,Zhang GL,Wang F

Tue Dec 26 00:00:00 EST 2017

Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Oncotarget

Igbe I,Shen XF,Jiao W,Qiang Z,Deng T,Li S,Liu WL,Liu HW,Zhang GL,Wang F

Tue Dec 26 00:00:00 EST 2017

Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation.

Cell death & disease

Lu L,Zhang S,Li C,Zhou C,Li D,Liu P,Huang M,Shen X

Thu May 11 00:00:00 EDT 2017

Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation.

Cell death & disease

Lu L,Zhang S,Li C,Zhou C,Li D,Liu P,Huang M,Shen X

Thu May 11 00:00:00 EDT 2017

Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells: Potential roles of suppressing STAT3 signaling.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Xiang M,Su H,Hong Z,Yang T,Shu G

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Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells: Potential roles of suppressing STAT3 signaling.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Xiang M,Su H,Hong Z,Yang T,Shu G

Mon Jun 01 00:00:00 EDT 2015

Expression and clinical significance of tyrosine phosphatase SHP2 in thyroid carcinoma.

Oncology letters

Hu ZQ,Ma R,Zhang CM,Li J,Li L,Hu ZT,Gao QI,Li WM

Tue Sep 01 00:00:00 EDT 2015

Expression and clinical significance of tyrosine phosphatase SHP2 in thyroid carcinoma.

Oncology letters

Hu ZQ,Ma R,Zhang CM,Li J,Li L,Hu ZT,Gao QI,Li WM

Tue Sep 01 00:00:00 EDT 2015

Expression and clinical significance of tyrosine phosphatase SHP2 in thyroid carcinoma.

Oncology letters

Hu ZQ,Ma R,Zhang CM,Li J,Li L,Hu ZT,Gao QI,Li WM

Tue Sep 01 00:00:00 EDT 2015

Expression and clinical significance of tyrosine phosphatase SHP2 in thyroid carcinoma.

Oncology letters

Hu ZQ,Ma R,Zhang CM,Li J,Li L,Hu ZT,Gao QI,Li WM

Tue Sep 01 00:00:00 EDT 2015

Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Hagberg N,Theorell J,Schlums H,Eloranta ML,Bryceson YT,Rönnblom L

Sun Sep 15 00:00:00 EDT 2013

Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells.

Journal of immunology (Baltimore, Md. : 1950)

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Sun Sep 15 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: encodes catalytic domain; HCP; HGNC:9644; MGC14433; Noonan syndrome 1; null; OTTHUMP00000166108; protein tyrosine phosphatase SH-PTP2; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; Protein-tyrosine phosphatase SYP; PTP-1D; PTP-2C; PTP1C; SH-PTP2; SH-PTP2 protein tyrosine phosphatase non-receptor type 11; SH-PTP2 protein tyrosine phosphatase, non-receptor type 11; SH-PTP3; SH2 domain-containing protein tyrosine phosphatase-2; SHP-2; Tyrosine-protein phosphatase non-receptor type 11

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Gene Aliases: 2700084A17Rik; AW536184; BPTP3; CFC; JMML; METCDS; NS1; PTP-1D; PTP1D; PTP2C; PTPN11; SAP-2; SH-PTP2; SH-PTP3; SHP-2; SHP2; SHPTP2; Syp

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UniProt ID: (Human) Q06124, (Mouse) Q64509, (Rat) P41499

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Entrez Gene ID: (Human) 5781, (Mouse) 19247, (Rat) 25622

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity non-membrane spanning protein tyrosine phosphatase activity SH3/SH2 adaptor activity insulin receptor binding protein binding 1-phosphatidylinositol-3-kinase activity protein domain specific binding receptor tyrosine kinase binding D1 dopamine receptor binding phospholipase binding insulin receptor substrate binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity peptide hormone receptor binding hydrolase activity phosphatase activity protein phosphatase

Process(es)

DNA damage checkpoint activation of MAPK activity triglyceride metabolic process epidermal growth factor receptor signaling pathway integrin-mediated signaling pathway axonogenesis brain development heart development fibroblast growth factor receptor signaling pathway hormone-mediated signaling pathway regulation of phosphatidylinositol 3-kinase signaling cerebellar cortex formation platelet activation platelet formation T cell costimulation microvillus organization abortive mitotic cell cycle regulation of cell adhesion mediated by integrin negative regulation of cell adhesion mediated by integrin multicellular organism growth organ growth peptidyl-tyrosine dephosphorylation megakaryocyte development phosphatidylinositol-3-phosphate biosynthetic process atrioventricular canal development ERBB signaling pathway regulation of multicellular organism growth hormone metabolic process glucose homeostasis regulation of protein complex assembly positive regulation of mitotic cell cycle negative regulation of insulin secretion regulation of protein export from nucleus phosphatidylinositol phosphorylation positive regulation of hormone secretion platelet-derived growth factor receptor signaling pathway neurotrophin TRK receptor signaling pathway ephrin receptor signaling pathway phosphatidylinositol-mediated signaling multicellular organismal reproductive process genitalia development inner ear development homeostasis of number of cells within a tissue leukocyte migration negative regulation of cortisol secretion Bergmann glial cell differentiation negative regulation of growth hormone secretion face morphogenesis regulation of type I interferon-mediated signaling pathway intestinal epithelial cell migration positive regulation of ERK1 and ERK2 cascade positive regulation of glucose import in response to insulin stimulus protein dephosphorylation lipid metabolic process positive regulation of signal transduction dephosphorylation negative regulation of hormone secretion

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SHP2 Polyclonal Antibody

SHP2 Antibody (20145-1-AP) in ICC/IF

Product Details

20145-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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