Invitrogen

SOCS1 Polyclonal Antibody

8 Published Figures

8 References

View all (16) SOCS1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (8)

SOCS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 13

SOCS1 Antibody (38-5200) in IHC (P)

Immunohistochemistry analysis of SOCS-1 showing staining in the cytoplasm and nucleus of paraffin-embedded human stomach carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at roo... View More

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Product Details

38-5200

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Insect, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Raised against the C-term of human SOCS1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533372

Target Information

The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins which regulate the immune cell responses to cytokines. SOCS1 acts to suppress dendritic cell (DC) as well as T cell hyperactivation following cytokine signaling by inhibiting JAK tyrosine kinase, a kinase necessary for type I and II cytokine receptors to initiate signaling, by directly binding to the catalytic domain of the kinase. SOCS1 also possesses E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome. It is through this method that SOCS1 negatively regulates signaling by Toll-like receptors TLR2 and TLR4 by mediating the degradation of the TLR signaling adaptor protein TIRAP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A mutual regulatory loop between miR-155 and SOCS1 influences renal inflammation and diabetic kidney disease.

Molecular therapy. Nucleic acids

Prieto I,Kavanagh M,Jimenez-Castilla L,Pardines M,Lazaro I,Herrero Del Real I,Flores-Muñoz M,Egido J,Lopez-Franco O,Gomez-Guerrero C

38-5200 was used in Western Blot to investigate the interplay between miR-155-5p and suppressor of cytokine signaling 1 (SOCS1) in the regulation of the JAK/STAT pathway during renal inflammation and diabetic kidney disease (DKD).

Tue Dec 12 00:00:00 EST 2023

A mutual regulatory loop between miR-155 and SOCS1 influences renal inflammation and diabetic kidney disease.

Molecular therapy. Nucleic acids

Prieto I,Kavanagh M,Jimenez-Castilla L,Pardines M,Lazaro I,Herrero Del Real I,Flores-Muñoz M,Egido J,Lopez-Franco O,Gomez-Guerrero C

Tue Dec 12 00:00:00 EST 2023

Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation.

Innate immunity

Bugueno IM,Benkirane-Jessel N,Huck O

Published figure using SOCS1 polyclonal antibody (Product # 38-5200) in Western Blot

Sat May 01 00:00:00 EDT 2021

N⁶-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.

Developmental cell

Du J,Liao W,Liu W,Deb DK,He L,Hsu PJ,Nguyen T,Zhang L,Bissonnette M,He C,Li YC

38-5200 was used in Western Blot to show that m6A methylation-mediated SOCS1 induction is required to maintain the negative feedback of macrophage activation in response to bacterial infection.

Mon Dec 21 00:00:00 EST 2020

Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment.

Oncology letters

Qin S,Gao Z,Liu Y,Liu C,Wang J,Zou LL

Published figure using SOCS1 polyclonal antibody (Product # 38-5200) in Western Blot

Thu Feb 01 00:00:00 EST 2018

Induction of miR-155 after Brain Injury Promotes Type 1 Interferon and has a Neuroprotective Effect.

Frontiers in molecular neuroscience

Harrison EB,Emanuel K,Lamberty BG,Morsey BM,Li M,Kelso ML,Yelamanchili SV,Fox HS

38-5200 was used in Western Blotting to examine the expression of inflammation associated miRNAs in the context of traumatic brain injury using a mouse controlled cortical impact model.

Wed Sep 30 00:00:00 EDT 2020

Signal Transducer and Activator of Transcription 1 (STAT1) Knock-down Induces Apoptosis in Malignant Pleural Mesothelioma.

Pathology oncology research : POR

Arzt L,Halbwedl I,Gogg-Kamerer M,Popper HH

385200 was used in immunohistochemistry - paraffin section and western blot to examine the oncogenic function of signal transducer and activator of transcription 1 in malignant pleural mesothelioma

Sat Jul 01 00:00:00 EDT 2017

1,25-Dihydroxyvitamin D promotes negative feedback regulation of TLR signaling via targeting microRNA-155-SOCS1 in macrophages.

Journal of immunology (Baltimore, Md. : 1950)

Chen Y,Liu W,Sun T,Huang Y,Wang Y,Deb DK,Yoon D,Kong J,Thadhani R,Li YC

38-5200 was used in western blot to demonstrate that vitamin D receptor signaling attenuates TLR-mediated inflammation.

Mon Apr 01 00:00:00 EDT 2013

Stage dependent aberrant regulation of cytokine-STAT signaling in murine systemic lupus erythematosus.

PloS one

Hale MB,Krutzik PO,Samra SS,Crane JM,Nolan GP

38-5200 was used in western blot to observe how the STAT family of transcription factors is altered during the progression of systemic lupus erythematosus

Tue Aug 25 00:00:00 EDT 2009

Stage dependent aberrant regulation of cytokine-STAT signaling in murine systemic lupus erythematosus.

PloS one

Hale MB,Krutzik PO,Samra SS,Crane JM,Nolan GP

38-5200 was used in western blot to observe how the STAT family of transcription factors is altered during the progression of systemic lupus erythematosus

Tue Aug 25 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: cytokine inducible SH2-containing protein 1; cytokine inducible SH2-containing protein 7; cytokine-inducible SH2 protein 1; JAB; JAK binding protein; JAK-binding protein; JAK2-binding protein; SOCS-1; SSI-1; STAT induced SH3 protein 1; STAT-induced STAT inhibitor 1; Suppressor of cytokine signaling 1; Tec-interacting protein 3; TIP-3

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Gene Aliases: CIS1; CISH1; Cish7; JAB; SOCS-1; SOCS1; SSI-1; SSI1; TIP3

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UniProt ID: (Human) O15524, (Mouse) O35716

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Entrez Gene ID: (Human) 8651, (Mouse) 12703

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Function(s)

protein kinase inhibitor activity insulin-like growth factor receptor binding protein binding kinase inhibitor activity protein kinase binding kinase modulator

Process(es)

regulation of protein phosphorylation negative regulation of protein kinase activity JAK-STAT cascade regulation of activation of JAK2 kinase activity protein ubiquitination cytokine-mediated signaling pathway organ regeneration response to estradiol response to lipopolysaccharide response to progesterone regulation of growth response to drug negative regulation of tyrosine phosphorylation of Stat1 protein negative regulation of tyrosine phosphorylation of Stat3 protein response to peptide hormone fat cell differentiation negative regulation of JAK-STAT cascade negative regulation of insulin receptor signaling pathway regulation of cytokine secretion regulation of interferon-gamma-mediated signaling pathway cellular response to amino acid stimulus negative regulation of signal transduction intracellular signal transduction regulation of tyrosine phosphorylation of Stat1 protein negative regulation of catalytic activity regulation of JAK-STAT cascade

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SOCS1 Polyclonal Antibody

SOCS1 Antibody (38-5200) in IHC (P)

Product Details

38-5200

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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