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STAT2 Antibody (701105) in IHC (P)
Immunohistochemistry analysis of STAT2 showing staining in the cytoplasm of paraffin-embedded mouse urinary bladder (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, ... View More
Product Details
701105
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1H10L19
Immunogen
Peptide corresponding to amino acids 186-197 of human singal transducer and activator of transcription 2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532390
Product Specific Information
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
STAT2 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: interferon alpha induced transcriptional activator; MGC59816; p113; Signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kDa; STAT; STAT 2; STAT-2
Gene Aliases: 1600010G07Rik; AW496480; IMD44; ISGF-3; P113; STAT113; STAT2
UniProt ID: (Human) P52630
Entrez Gene ID: (Human) 6773, (Mouse) 20847
regulation of protein phosphorylation
positive regulation of defense response to virus by host
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
JAK-STAT cascade
defense response to virus
type I interferon signaling pathway
regulation of type I interferon-mediated signaling pathway
regulation of mitochondrial fission
mitophagy in response to mitochondrial depolarization
xenophagy
regulation of transcription, DNA-templated
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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