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TAF15 Antibody (PA5-106521) in ICC/IF
Immunofluorescent analysis of TAF15 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with TAF15 polyclonal antibody (Product # PA5-106521) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-106521
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TAF15(Accession Q92804), corresponding to amino acid residues C360-G410.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854190
Product Specific Information
Antibody detects endogenous levels of total TAF15.
Target Information
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 68 kDa TATA-binding protein-associated factor; hTAFII68; RBP56/CSMF fusion; RNA-binding protein 56; TAF(II)68; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68 kDa; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa; TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA binding protein 56); TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56); TATA box binding protein associated factor 15; TATA box-binding protein-associated factor 2N (RNA-binding protein 56); TATA-bin; TATA-binding protein-associated factor 2N; TBP-associated factor 15
Gene Aliases: 2610111C21Rik; 68kDa; Npl3; RBP56; TAF15; TAF2N; TAFII68
UniProt ID: (Human) Q92804
Entrez Gene ID: (Human) 8148, (Mouse) 70439, (Rat) 287571
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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