Proteintech

TAK1 Polyclonal Antibody

TAK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 22

TAK1 Antibody (12330-2-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using TAK1 antibody (Product # 12330-2-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

TAK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

12330-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 33 publications 33 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:200

Immunoprecipitation (IP)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Hamster, Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TAK1 Fusion Protein Ag3041 (1-291 aa encoded by BC017715)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSTASAASS SSSSSAGEMI EAPSQVLNFE EIDYKEIEVE EVVGRGAFGV VCKAKWRAKD VAIKQIESES ERKAFIVELR QLSRVNHPNI VKLYGACLNP VCLVMEYAEG GSLYNVLHGA EPLPYYTAAH AMSWCLQCSQ GVAYLHSMQP KALIHRDLKP PNLLLVAGGT VLKICDFGTA CDIQTHMTNN KGSAAWMAPE VFEGSNYSEK CDVFSWGIIL WEVITRRKPF DEIGGPAFRI MWAVHNGTRP PLIKNLPKPI ESLMTRCWSK DPSQRPSMEE IVKIMTHLMR YF (1-291 aa encoded by BC017715)

Target Information

TAK1 (also known as MAP3K7), a part of the serine/threonine kinase family, is a versatile protein with many signaling functions. Originally identified as a TGF- beta-activated kinase, TAK1 interacts with TGF- beta-receptor and TRAF6 to modulate TGF- beta activation of JNK and p38. It has additional roles in activation of p38 and JNK through Wnt, BMP, and activin signaling pathways as well in response to thyroid hormone, osmotic stress, endothelin, and ephrine. Through activation of von Hippel-Lindau tumor suppressor expression, TAK1 represses PDGF-B, integrin beta1 and integrin beta5, promoting proper wound healing. TAK1 also plays an essential role in IKK activation in numerous signaling pathways including IL-1, IL-6, IL-18, TNF, CD40, TLR, and RIG-I. Activation of the TAK1-IKK pathway requires TAB2/3 and ubiquitination. In this process, TAB2/3 binds to the C-terminal region of TAK1 and becomes polyubiquitinated, TAK1 is autophosphorylated at Thr178, Thr184, Thr187 and Ser192, and finally TAK1 phosphorylates IKK- beta. Additionally, TAK1 represses human telomerase reverse transcriptase suggesting a role in regulation of cell lifespan.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: M3K7; mitogen activated protein kinase kinase kinase 7; Mitogen-activated protein kinase kinase kinase 7; RP1-154G14.1; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; transforming growth factor beta-activated kinase 1; Transforming growth factor-beta-activated kinase 1

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Gene Aliases: B430101B05; C87327; I79_000697; MAP3K7; MEKK7; TAK1; TGF1a

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UniProt ID: (Human) O43318, (Mouse) Q62073

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Entrez Gene ID: (Human) 6885, (Mouse) 26409, (Chinese hamster) 100774341

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Function(s)

magnesium ion binding protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding scaffold protein binding nucleotide binding MAP kinase kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding non-receptor serine/threonine protein kinase protein modifying enzyme

Process(es)

activation of MAPKK activity activation of MAPK activity stimulatory C-type lectin receptor signaling pathway positive regulation of T cell cytokine production MyD88-dependent toll-like receptor signaling pathway transcription, DNA-templated apoptotic process transforming growth factor beta receptor signaling pathway Wnt signaling pathway, calcium modulating pathway I-kappaB kinase/NF-kappaB signaling activation of NF-kappaB-inducing kinase activity I-kappaB phosphorylation JNK cascade positive regulation of interleukin-2 production Fc-epsilon receptor signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of JUN kinase activity histone H3 acetylation T cell receptor signaling pathway positive regulation of T cell activation positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade nucleotide-binding oligomerization domain containing signaling pathway MAPK cascade angiogenesis neural tube formation regulation of transcription, DNA-templated protein phosphorylation phosphorylation positive regulation of JNK cascade negative regulation of necroptotic process negative regulation of ripoptosome assembly involved in necroptotic process regulation of reactive oxygen species metabolic process negative regulation of reactive oxygen species metabolic process negative regulation of apoptotic signaling pathway

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TAK1 Polyclonal Antibody

TAK1 Antibody (12330-2-AP) in ICC/IF

Product Details

12330-2-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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