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TBK1 Antibody (702283) in ICC/IF
Immunofluorescence analysis of TBK1 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TBK1 Recombinant Rabbit Monoclonal Antibody (10H15L13) (Product # 702283) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Flu... View More
Product Details
702283
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
10H15L13
Immunogen
Peptides corresponding to Human TBK1 (aa 480-497, 260-272)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633077
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Bovine and Dog
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
TBK1 serine/threonine kinase is a member of the IKK family and is involved in NF-kappa B activation and IRF3 signaling. TBK1 is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-KB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-KB is associated with IKB proteins in the cell cytoplasm, which inhibit NF-KB activity. Phosphorylation of I-KB by IKB kinase (IKK) complex leads to degradation of I-KB and activation of NF-KB. The IKK complex contains IKKa, IKK beta, and IKKK. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-KB-activating kinase), and T2K. NAK/TBK1 activates IKK beta through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ11330; NF-kappa-B-activating kinase; NF-kB-activating kinase; serine/threonine protein kinase TBK1; Serine/threonine-protein kinase TBK1; T2K; TANK-binding kinase 1; tbk
Gene Aliases: 1200008B05Rik; AI462036; AW048562; FTDALS4; NAK; T2K; TBK1
UniProt ID: (Human) Q9UHD2, (Mouse) Q9WUN2
Entrez Gene ID: (Human) 29110, (Mouse) 56480, (Rat) 299827
nucleic acid binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
phosphoprotein binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
inflammatory response
I-kappaB kinase/NF-kappaB signaling
response to virus
negative regulation of gene expression
peptidyl-serine phosphorylation
negative regulation of type I interferon production
positive regulation of type I interferon production
type I interferon production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of peptidyl-serine phosphorylation
TRIF-dependent toll-like receptor signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
dendritic cell proliferation
innate immune response
positive regulation of interferon-beta biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
defense response to Gram-positive bacterium
defense response to virus
regulation of neuron death
positive regulation of xenophagy
activation of innate immune response
immune system process
protein phosphorylation
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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