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TBK1 Antibody (MA5-50051) in IHC (P)
Immunohistochemistry analysis with a TBK1 monoclonal antibody (Product # MA5-50051) using a dilution of 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incuba... View More
Product Details
MA5-50051
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
14D10
Immunogen
A synthesized peptide derived from human TBK1
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_3092541
Target Information
TBK1 serine/threonine kinase is a member of the IKK family and is involved in NF-kappa B activation and IRF3 signaling. TBK1 is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-KB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-KB is associated with IKB proteins in the cell cytoplasm, which inhibit NF-KB activity. Phosphorylation of I-KB by IKB kinase (IKK) complex leads to degradation of I-KB and activation of NF-KB. The IKK complex contains IKKa, IKK beta, and IKKK. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-KB-activating kinase), and T2K. NAK/TBK1 activates IKK beta through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ11330; NF-kappa-B-activating kinase; NF-kB-activating kinase; Serine/threonine-protein kinase TBK1; T2K; TANK-binding kinase 1; tbk
Gene Aliases: FTDALS4; NAK; T2K; TBK1
UniProt ID: (Human) Q9UHD2
Entrez Gene ID: (Human) 29110
inflammatory response
I-kappaB kinase/NF-kappaB signaling
response to virus
negative regulation of gene expression
peptidyl-serine phosphorylation
negative regulation of type I interferon production
positive regulation of type I interferon production
type I interferon production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of peptidyl-serine phosphorylation
TRIF-dependent toll-like receptor signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
dendritic cell proliferation
innate immune response
positive regulation of interferon-beta biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
defense response to Gram-positive bacterium
defense response to virus
regulation of neuron death
positive regulation of xenophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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