Proteintech

TCF4 Polyclonal Antibody

TCF4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TCF4 Antibody (22337-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human testis slide using 22337-1-AP ( TCF4 Antibody) at dilution of 1:200.

TCF4 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

TCF4 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

22337-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 35 publications 35 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:200

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 4 publications 4 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fruit fly, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TCF4 Fusion Protein Ag17813 (407-560 aa encoded by BC125084)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: PSTAMPGGH GDMHGIIGPS HNGAMGGLGS GYGTGLLSAN RHSLMVGTHR EDGVALRGSH SLLPNQVPVP QLPVQSATSP DLNPPQDPYR GMPPGLQGQS VSSGSSEIKS DDEGDENLQD TKSSEDKKLD DDKKDIKSIT RSRSSNNDDE DLTPE (407-560 aa encoded by BC125084)

Target Information

TCF4 is transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG')-a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Defects in this gene are a cause of Pitt-Hopkins syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Fri Feb 17 00:00:00 EST 2023

B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin.

Signal transduction and targeted therapy

Zhou C,Zhu X,Liu N,Dong X,Zhang X,Huang H,Tang Y,Liu S,Hu M,Wang M,Deng X,Li S,Zhang R,Huang Y,Lyu H,Xiao S,Luo S,Ali DW,Michalak M,Chen XZ,Wang Z,Tang J

Fri Feb 17 00:00:00 EST 2023

lncRNA MEG3 Promotes Osteogenic Differentiation of Tendon Stem Cells Via the miR-129-5p/TCF4/β-Catenin Axis and thus Contributes to Trauma-Induced Heterotopic Ossification.

Stem cell reviews and reports

Liu H,Sun Z,Luo G,Hu Y,Ruan H,Tu B,Li J,Fan C

Sun Oct 01 00:00:00 EDT 2023

Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity.

iScience

He T,Wang S,Li S,Shen H,Hou L,Liu Y,Wei Y,Xie F,Zhang Z,Zhao Z,Mo C,Guo H,Huang Q,Zhang R,Shen D,Li B

Fri Apr 21 00:00:00 EDT 2023

Ursolic Acid Suppresses Colorectal Cancer by Down-Regulation of Wnt/β-Catenin Signaling Pathway Activity.

Journal of agricultural and food chemistry

Zhao H,Tang S,Tao Q,Ming T,Lei J,Liang Y,Peng Y,Wang M,Liu M,Yang H,Ren S,Xu H

Wed Mar 08 00:00:00 EST 2023

Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.

Journal of translational medicine

Liu X,Jiang L,Zhang W,Zhang J,Luan X,Zhan Y,Wang T,Da J,Liu L,Zhang S,Guo Y,Zhang K,Wang Z,Miao N,Xie X,Liu P,Li Y,Jin H,Zhang B

Tue Jun 27 00:00:00 EDT 2023

Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.

Journal of translational medicine

Liu X,Jiang L,Zhang W,Zhang J,Luan X,Zhan Y,Wang T,Da J,Liu L,Zhang S,Guo Y,Zhang K,Wang Z,Miao N,Xie X,Liu P,Li Y,Jin H,Zhang B

Tue Jun 27 00:00:00 EDT 2023

Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.

Journal of translational medicine

Liu X,Jiang L,Zhang W,Zhang J,Luan X,Zhan Y,Wang T,Da J,Liu L,Zhang S,Guo Y,Zhang K,Wang Z,Miao N,Xie X,Liu P,Li Y,Jin H,Zhang B

Tue Jun 27 00:00:00 EDT 2023

Targeting lncRNA DDIT4-AS1 Sensitizes Triple Negative Breast Cancer to Chemotherapy via Suppressing of Autophagy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Jiang T,Zhu J,Jiang S,Chen Z,Xu P,Gong R,Zhong C,Cheng Y,Sun X,Yi W,Yang J,Zhou W,Cheng Y

Thu Jun 01 00:00:00 EDT 2023

Oridonin promotes endoplasmic reticulum stress via TP53-repressed TCF4 transactivation in colorectal cancer.

Journal of experimental & clinical cancer research : CR

Zhou F,Gao H,Shang L,Li J,Zhang M,Wang S,Li R,Ye L,Yang S

Mon Jun 19 00:00:00 EDT 2023

Integrative network analysis of circular RNAs reveals regulatory mechanisms for hepatic specification of human iPSC-derived endoderm.

Stem cell research & therapy

Bai F,Duan J,Yang D,Lai X,Zhu X,He X,Hu A

Thu Sep 08 00:00:00 EDT 2022

Regulation and function of Id2 in plasmacytoid dendritic cells.

Molecular immunology

Babcock RL,Zhou Y,Patel B,Chrisikos TT,Kahn LM,Dyevoich AM,Medik YB,Watowich SS

Mon Aug 01 00:00:00 EDT 2022

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Nature

Burda JE,O'Shea TM,Ao Y,Suresh KB,Wang S,Bernstein AM,Chandra A,Deverasetty S,Kawaguchi R,Kim JH,McCallum S,Rogers A,Wahane S,Sofroniew MV

Wed Jun 01 00:00:00 EDT 2022

miR-145-5p targets MMP2 to protect brain injury in hypertensive intracerebral hemorrhage via inactivation of the Wnt/β-catenin signaling pathway.

Annals of translational medicine

Xiao W,Jiang Z,Wan W,Pan W,Xu J

Sun May 01 00:00:00 EDT 2022

Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes.

Nature communications

Lehman NL,Spassky N,Sak M,Webb A,Zumbar CT,Usubalieva A,Alkhateeb KJ,McElroy JP,Maclean KH,Fadda P,Liu T,Gangalapudi V,Carver J,Abdullaev Z,Timmers C,Parker JR,Pierson CR,Mobley BC,Gokden M,Hattab EM,Parrett T,Cooke RX,Lehman TD,Costinean S,Parwani A,Williams BJ,Jensen RL,Aldape K,Mistry AM

Tue Apr 19 00:00:00 EDT 2022

Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model.

Evidence-based complementary and alternative medicine : eCAM

Yang X,Luo G,Fu L,Huang H,Wang L,Yin L,Zhang X,Wang T,Ma X,Feng T,Ye J

Sat Jul 16 00:00:00 EDT 2022

Inhibition of GABA_AR or Application of Lactobacillus casei Zhang Alleviates Ulcerative Colitis in Mice: GABA_AR as a Potential Target for Intestinal Epithelial Renewal and Repair.

International journal of molecular sciences

Wang Q,Deng Z,Lan J,Li D,Fan K,Chang J,Ma Y

Fri Sep 23 00:00:00 EDT 2022

ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer.

Journal of experimental & clinical cancer research : CR

Dong S,Liang S,Cheng Z,Zhang X,Luo L,Li L,Zhang W,Li S,Xu Q,Zhong M,Zhu J,Zhang G,Hu S

Sat Jan 08 00:00:00 EST 2022

ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer.

Journal of experimental & clinical cancer research : CR

Dong S,Liang S,Cheng Z,Zhang X,Luo L,Li L,Zhang W,Li S,Xu Q,Zhong M,Zhu J,Zhang G,Hu S

Sat Jan 08 00:00:00 EST 2022

Mannan-binding lectin exacerbates the severity of psoriasis by promoting plasmacytoid dendritic cell differentiation via the signal transducer and activator of transcription 3-interferon regulatory factor 8 axis.

The Journal of dermatology

Zeng J,Wang D,Luo J,Li L,Lin L,Li J,Zheng W,Zuo D,Yang B

Sun May 01 00:00:00 EDT 2022

Matrine inhibits the Wnt3a/β-catenin/TCF7L2 signaling pathway in experimental autoimmune encephalomyelitis.

Journal of neuroimmunology

Ma R,Chu Y,Dou M,Jing Y,Zhao X,Li X,Li S,Zhang Y,Wang L,Zhu L

Wed Jun 15 00:00:00 EDT 2022

AR-regulated ZIC5 contributes to the aggressiveness of prostate cancer.

Cell death discovery

Tan YF,Zhang Y,Ge SY,Zhong F,Sun CY,Xia GW

Tue Sep 20 00:00:00 EDT 2022

AR-regulated ZIC5 contributes to the aggressiveness of prostate cancer.

Cell death discovery

Tan YF,Zhang Y,Ge SY,Zhong F,Sun CY,Xia GW

Tue Sep 20 00:00:00 EDT 2022

The neuroprotective mechanism of lithium after ischaemic stroke.

Communications biology

Chen B,Zhang M,Ji M,Zhang D,Chen B,Gong W,Li X,Zhou Y,Dong C,Wen G,Zhan X,Wu X,Yuan H,Xu E,Xia M,Verkhratsky A,Li B

Thu Feb 03 00:00:00 EST 2022

The neuroprotective mechanism of lithium after ischaemic stroke.

Communications biology

Chen B,Zhang M,Ji M,Zhang D,Chen B,Gong W,Li X,Zhou Y,Dong C,Wen G,Zhan X,Wu X,Yuan H,Xu E,Xia M,Verkhratsky A,Li B

Thu Feb 03 00:00:00 EST 2022

A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation.

Nature communications

Wu J,Yang K,Cai S,Zhang X,Hu L,Lin F,Wu SQ,Xiao C,Liu WH,Han J

Mon Nov 28 00:00:00 EST 2022

regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.

American journal of physiology. Heart and circulatory physiology

Lin L,Xu W,Li Y,Zhu P,Yuan W,Liu M,Shi Y,Chen Y,Liang J,Chen J,Yang B,Cai W,Wen Y,Zhu X,Peng X,Zhou Z,Mo X,Wan Y,Yuan H,Li F,Ye X,Jiang Z,Wang Y,Zhuang J,Fan X,Wu X

Thu Apr 01 00:00:00 EDT 2021

TCF4 and HuR mediated-METTL14 suppresses dissemination of colorectal cancer via N6-methyladenosine-dependent silencing of ARRDC4.

Cell death & disease

Wang H,Wei W,Zhang ZY,Liu Y,Shi B,Zhong W,Zhang HS,Fang X,Sun CL,Wang JB,Liu LX

Fri Dec 17 00:00:00 EST 2021

Scutellarin ameliorates colitis-associated colorectal cancer by suppressing Wnt/β-catenin signaling cascade.

European journal of pharmacology

Zeng S,Chen L,Sun Q,Zhao H,Yang H,Ren S,Liu M,Meng X,Xu H

Sun Sep 05 00:00:00 EDT 2021

FHL1 Inhibits the Progression of Colorectal Cancer by Regulating the Wnt/β-Catenin Signaling Pathway.

Journal of Cancer

Liu Y,Wang C,Cheng P,Zhang S,Zhou W,Xu Y,Xu H,Ji G

Tue Aug 03 00:00:00 EDT 2021

Circ_SMAD4 promotes gastric carcinogenesis by activating wnt/β-catenin pathway.

Cell proliferation

Wang L,Li B,Yi X,Xiao X,Zheng Q,Ma L

Mon Mar 01 00:00:00 EST 2021

A positive feedback loop of lncRNA-RMRP/ZNRF3 axis and Wnt/β-catenin signaling regulates the progression and temozolomide resistance in glioma.

Cell death & disease

Liu T,Hu J,Han B,Tan S,Jia W,Xin Y

Sat Oct 16 00:00:00 EDT 2021

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

Acta pharmaceutica Sinica. B

Wu X,Liu L,Zheng Q,Hao H,Ye H,Li P,Yang H

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Mouse Retinal Cell Atlas: Molecular Identification of over Sixty Amacrine Cell Types.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Yan W,Laboulaye MA,Tran NM,Whitney IE,Benhar I,Sanes JR

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NFE2L3 promotes malignant behavior and EMT of human hepatocellular carcinoma (HepG2) cells via Wnt/β‑catenin pathway.

Journal of Cancer

Ren Y,Wang Y,Hao S,Yang Y,Xiong W,Qiu L,Tao J,Tang A

Sat Oct 31 00:00:00 EDT 2020

Linc00261 inhibits metastasis and the WNT signaling pathway of pancreatic cancer by regulating a miR‑552‑5p/FOXO3 axis.

Oncology reports

Chen T,Lei S,Zeng Z,Zhang J,Xue Y,Sun Y,Lan J,Xu S,Mao D,Guo B

Sun Mar 01 00:00:00 EST 2020

MicroRNA‑137 suppresses the proliferation, migration and invasion of cholangiocarcinoma cells by targeting WNT2B.

International journal of molecular medicine

Chen T,Lei S,Zeng Z,Pan S,Zhang J,Xue Y,Sun Y,Lan J,Xu S,Mao D,Guo B

Sun Mar 01 00:00:00 EST 2020

miR-129-5p Inhibits Bone Formation Through TCF4.

Frontiers in cell and developmental biology

Yin C,Tian Y,Yu Y,Yang C,Su P,Zhao Y,Wang X,Zhang K,Pei J,Li D,Chen Z,Zhang Y,Miao Z,Qian A

Fri Nov 27 00:00:00 EST 2020

miR-129-5p Inhibits Bone Formation Through TCF4.

Frontiers in cell and developmental biology

Yin C,Tian Y,Yu Y,Yang C,Su P,Zhao Y,Wang X,Zhang K,Pei J,Li D,Chen Z,Zhang Y,Miao Z,Qian A

Fri Nov 27 00:00:00 EST 2020

ADAR1 may be involved in the proliferation of acute myeloid leukemia cells via regulation of the Wnt pathway.

Cancer management and research

Xiao H,Cheng Q,Wu X,Tang Y,Liu J,Li X

Sun Apr 10 00:00:00 EDT 2022

XRCC4, which is inhibited by PFDA, regulates DNA damage repair and cell chemosensitivity.

Journal of cellular biochemistry

Liu F,Fan Z,Song N,Han M,Yan M,Guo LH,Jihui J,Liu S

Thu Aug 01 00:00:00 EDT 2019

Ursolic acid promotes apoptosis and mediates transcriptional suppression of CT45A2 gene expression in non-small-cell lung carcinoma harbouring EGFR T790M mutations.

British journal of pharmacology

Yang K,Chen Y,Zhou J,Ma L,Shan Y,Cheng X,Wang Y,Zhang Z,Ji X,Chen L,Dai H,Zhu B,Li C,Tao Z,Hu X,Yin W

Sun Dec 01 00:00:00 EST 2019

Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells.

Journal of ginseng research

Wang X,Su GY,Zhao C,Qu FZ,Wang P,Zhao YQ

Sun Apr 01 00:00:00 EDT 2018

Wenyang Huazhuo Tongluo formula inhibits fibrosis via suppressing Wnt/β-catenin signaling pathway in a Bleomycin-induced systemic sclerosis mouse model.

Chinese medicine

Wang Q,Zang W,Han L,Yang L,Ye S,Ouyang J,Zhang C,Bi Y,Zhang C,Bian H

Fri Apr 08 00:00:00 EDT 2022

TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.

Cancer letters

Liu SL,Lin HX,Lin CY,Sun XQ,Ye LP,Qiu F,Wen W,Hua X,Wu XQ,Li J,Song LB,Guo L

Mon Aug 28 00:00:00 EDT 2017

Tcf4 Controls Neuronal Migration of the Cerebral Cortex through Regulation of Bmp7.

Frontiers in molecular neuroscience

Chen T,Wu Q,Zhang Y,Lu T,Yue W,Zhang D

Wed Sep 30 00:00:00 EDT 2020

Tcf4 Controls Neuronal Migration of the Cerebral Cortex through Regulation of Bmp7.

Frontiers in molecular neuroscience

Chen T,Wu Q,Zhang Y,Lu T,Yue W,Zhang D

Wed Sep 30 00:00:00 EDT 2020

Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons.

Cell reports

D'Rozario M,Zhang T,Waddell EA,Zhang Y,Sahin C,Sharoni M,Hu T,Nayal M,Kutty K,Liebl F,Hu W,Marenda DR

Tue Apr 12 00:00:00 EDT 2016

Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons.

Cell reports

D'Rozario M,Zhang T,Waddell EA,Zhang Y,Sahin C,Sharoni M,Hu T,Nayal M,Kutty K,Liebl F,Hu W,Marenda DR

Tue Apr 12 00:00:00 EDT 2016

Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons.

Cell reports

D'Rozario M,Zhang T,Waddell EA,Zhang Y,Sahin C,Sharoni M,Hu T,Nayal M,Kutty K,Liebl F,Hu W,Marenda DR

Tue Apr 12 00:00:00 EDT 2016

Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons.

Cell reports

D'Rozario M,Zhang T,Waddell EA,Zhang Y,Sahin C,Sharoni M,Hu T,Nayal M,Kutty K,Liebl F,Hu W,Marenda DR

Tue Apr 12 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: bHLHb19; Class A helix-loop-helix transcription factor ME2; Class B basic helix-loop-helix protein 19; E2 2; Immunoglobulin transcription factor 2; immunoglobulin transcription factor-2; ITF 2; ITF-2; MGC149723; MGC149724; MITF-2; SEF 2; SEF-2; SEF2 1; SEF2 1A; SEF2 1B; SL3-3 enhancer factor 2; TCF 4; TCF-4; Transcription factor 4; Transcription factor 4 (Immunoglobulin transcription factor 2) (ITF-2) (MITF-2) (SL3-3 enhancer factor 2) (SEF-2) (Class A helix-loop-helix transcription factor ME2); transcription factor 4, isoform C; transcription factor 4, isoform D; transcription factor 4, isoform E; transcription factor 4, isoform L; transcription factor 4, isoform M; transcription factor 4, isoform R

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Gene Aliases: 5730422P05Rik; ASP-I2; BHLHB19; E2-2; E2.2; FECD3; ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-4; TCF4; TFE

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UniProt ID: (Human) P15884, (Mouse) Q60722

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Entrez Gene ID: (Human) 6925, (Mouse) 21413

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding, RNA polymerase recruiting transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, TFIIB-class binding TFIIB-class transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein C-terminus binding identical protein binding protein homodimerization activity bHLH transcription factor binding protein heterodimerization activity E-box binding chromatin binding double-stranded DNA binding transcription corepressor activity protein dimerization activity basic helix-loop-helix transcription factor

Process(es)

DNA-templated transcription, initiation transcription initiation from RNA polymerase II promoter positive regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter protein-DNA complex assembly negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development nervous system development cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TCF4 Polyclonal Antibody

TCF4 Antibody (22337-1-AP) in IHC (P)

Product Details

22337-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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