Proteintech

TCOF1 Polyclonal Antibody

TCOF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

TCOF1 Antibody (11003-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using TCOF1 antibody 11003-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

TCOF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

11003-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TCOF1 Fusion Protein Ag1447 (1-350 aa encoded by BC014559)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAEARKRRE LLPLIYHHLL RAGYVRAARE VKEQSGQKCF LAQPVTLLDI YTHWQQTSEL GRKRKAEEDA ALQAKKTRVS DPISTSESSE EEEEAEAETA KATPRLASTN SSVLGADLPS SMKEKAKAET EKAGKTGNSM PHPATGKTVA NLLSGKSPRK SAEPSANTTL VSETEEEGSV PAFGAAAKPG MVSAGQADSS SEDTSSSSDE TDVEGKPSVK PAQVKASSVS TKESPARKAA PAPGKVGDVT PQVKGGALPP AKRAKKPEEE SESSEEGSES EEEAPAGTRS QVKASEKILQ VRAASAPAKG TPGKGATPAP PGKAGAVASQ TKAGKPEEDS ESSSEESSDS E (1-350 aa encoded by BC014559)

Target Information

Treacle is a serine/alanine-rich 1,411 amino acid nuclear phosphoprotein that interacts with upstream binding factor (UBF) and affects transcription of the ribosomal DNA gene. Treacle is also involved in early embryonic development, particularly in the craniofacial complex, and may play a role in nucleolarcytoplasmic transport. The Treacle protein contains three domains with unique N- and C-termini and a large central repeat domain. Mutations in TCOF1, the gene that encodes for Treacle, cause Treacher Collins-Franceschetti syndrome (TCS), a disorder characterized by defects in craniofacial development. Symptoms of TCS include conductive hearing loss, hypoplasia of the mandible and maxilla, downward sloping palpebral fissures and cleft palate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Henipaviruses and lyssaviruses target nucleolar treacle protein and regulate ribosomal RNA synthesis.

Traffic (Copenhagen, Denmark)

Rawlinson SM,Zhao T,Ardipradja K,Zhang Y,Veugelers PF,Harper JA,David CT,Sundaramoorthy V,Moseley GW

Wed Mar 01 00:00:00 EST 2023

TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.

Cancer science

Wu C,Xia D,Wang D,Wang S,Sun Z,Xu B,Zhang D

Tue Feb 01 00:00:00 EST 2022

TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.

Cancer science

Wu C,Xia D,Wang D,Wang S,Sun Z,Xu B,Zhang D

Tue Feb 01 00:00:00 EST 2022

TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.

International journal of molecular sciences

Hassan A,Iftinca M,Young D,Flynn R,Agosti F,Abdullah N,Defaye M,Scott MGH,Dufour A,Altier C

Thu Feb 25 00:00:00 EST 2021

TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.

International journal of molecular sciences

Hassan A,Iftinca M,Young D,Flynn R,Agosti F,Abdullah N,Defaye M,Scott MGH,Dufour A,Altier C

Thu Feb 25 00:00:00 EST 2021

Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.

eLife

Werner A,Baur R,Teerikorpi N,Kaya DU,Rape M

Thu Jul 12 00:00:00 EDT 2018

Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.

eLife

Werner A,Baur R,Teerikorpi N,Kaya DU,Rape M

Thu Jul 12 00:00:00 EDT 2018

Viral regulation of host cell biology by hijacking of the nucleolar DNA-damage response.

Nature communications

Rawlinson SM,Zhao T,Rozario AM,Rootes CL,McMillan PJ,Purcell AW,Woon A,Marsh GA,Lieu KG,Wang LF,Netter HJ,Bell TDM,Stewart CR,Moseley GW

Fri Aug 03 00:00:00 EDT 2018

Viral regulation of host cell biology by hijacking of the nucleolar DNA-damage response.

Nature communications

Rawlinson SM,Zhao T,Rozario AM,Rootes CL,McMillan PJ,Purcell AW,Woon A,Marsh GA,Lieu KG,Wang LF,Netter HJ,Bell TDM,Stewart CR,Moseley GW

Fri Aug 03 00:00:00 EDT 2018

Cell-fate determination by ubiquitin-dependent regulation of translation.

Nature

Werner A,Iwasaki S,McGourty CA,Medina-Ruiz S,Teerikorpi N,Fedrigo I,Ingolia NT,Rape M

Thu Sep 24 00:00:00 EDT 2015

Cell-fate determination by ubiquitin-dependent regulation of translation.

Nature

Werner A,Iwasaki S,McGourty CA,Medina-Ruiz S,Teerikorpi N,Fedrigo I,Ingolia NT,Rape M

Thu Sep 24 00:00:00 EDT 2015

Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.

Proceedings of the National Academy of Sciences of the United States of America

Ciccia A,Huang JW,Izhar L,Sowa ME,Harper JW,Elledge SJ

Tue Dec 30 00:00:00 EST 2014

Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.

Proceedings of the National Academy of Sciences of the United States of America

Ciccia A,Huang JW,Izhar L,Sowa ME,Harper JW,Elledge SJ

Tue Dec 30 00:00:00 EST 2014

Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.

Proceedings of the National Academy of Sciences of the United States of America

Ciccia A,Huang JW,Izhar L,Sowa ME,Harper JW,Elledge SJ

Tue Dec 30 00:00:00 EST 2014

Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.

Proceedings of the National Academy of Sciences of the United States of America

Ciccia A,Huang JW,Izhar L,Sowa ME,Harper JW,Elledge SJ

Tue Dec 30 00:00:00 EST 2014

CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function.

PloS one

Park A,Won ST,Pentecost M,Bartkowski W,Lee B

Tue Dec 30 00:00:00 EST 2014

CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function.

PloS one

Park A,Won ST,Pentecost M,Bartkowski W,Lee B

Tue Dec 30 00:00:00 EST 2014

CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function.

PloS one

Park A,Won ST,Pentecost M,Bartkowski W,Lee B

Tue Dec 30 00:00:00 EST 2014

CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function.

PloS one

Park A,Won ST,Pentecost M,Bartkowski W,Lee B

Tue Dec 30 00:00:00 EST 2014

Bioinformatics

Protein Aliases: nucleolar trafficking phosphoprotein; Treacher Collins syndrome protein; Treacher Collins syndrome protein homolog; Treacher Collins-Franceschetti syndrome 1; Treacle protein

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Gene Aliases: AA408847; AW209012; MFD1; TCOF1; TCS; TCS1; treacle

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UniProt ID: (Human) Q13428, (Mouse) O08784

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Entrez Gene ID: (Human) 6949, (Mouse) 21453

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Function(s)

transporter activity protein binding poly(A) RNA binding RNA metabolism protein

Process(es)

skeletal system development transport transcription of nuclear large rRNA transcript from RNA polymerase I promoter

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TCOF1 Polyclonal Antibody

TCOF1 Antibody (11003-1-AP) in ICC/IF

Product Details

11003-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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