Proteintech

TNFAIP3 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

23456-1-AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 15 publications 15 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TNFAIP3 Fusion Protein Ag18150 (441-790 aa encoded by BC114480)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody, which is a rabbit polyclonal antibody raised against 350 aa of C-terminus of human TNFAIP3, can recognize full-length A20 (~90 kDa) and cleaved form (~37-40 kDa).

Immunogen sequence: EAYEPLAWN PEESTGGPHS APPTAPSPFL FSETTAMKCR SPGCPFTLNV QHNGFCERCH NARQLHASHA PDHTRHLDPG KCQACLQDVT RTFNGICSTC FKRTTAEASS SLSTSLPPSC HQRSKSDPSR LVRSPSPHSC HRAGNDAPAG CLSQAARTPG DRTGTSKCRK AGCVYFGTPE NKGFCTLCFI EYRENKHFAA ASGKVSPTAS RFQNTIPCLG RECGTLGSTM FEGYCQKCFI EAQNQRFHEA KRTEEQLRSS QRRDVPRTTQ STSRPKCARA SCKNILACRS EELCMECQHP NQRMGPGAHR GEPAPEDPPK QRCRAPACDH FGNAKCNGYC NECFQFKQMY G (441-790 aa encoded by BC114480)

Target Information

TNFAIP3, also known as A20, is located in chromosome band 6q23, a region that is often deleted in B cell lymphomas. Recently, it was identified as a tumor suppressor gene in Hodgkin lymphoma and several subtypes of non-Hodgkin lymphomas. TNFAIP3 was initially identified as a zinc-finger protein that is rapidly and transiently induced by TNF-a, inhibiting NF-kappa-B-dependent gene expression, and protecting cells from TNF-a-cytotoxicity. Overexpression of TNFAIP3 also inhibits the TLR2- and TLR4-mediated interleukin-8 synthesis in airway epithelial cells, suggesting that TNFAIP3 also acts as a negative regulator of TLR-mediated inflammatory responses, thereby protecting the host against harmful over-responses to pathogens. At least two isoforms of TNFAIP3 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: OTU domain-containing protein 7C; Putative DNA-binding protein A20; RP11-356I2.3; TNF alpha-induced protein 3; TNFAIP3 (A20); Tumor necrosis factor alpha-induced protein 3; tumor necrosis factor induced protein 3; tumor necrosis factor inducible protein A20; tumor necrosis factor, alpha induced protein 3; tumor necrosis factor, alpha-induced protein 3; Zinc finger protein A20

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Gene Aliases: A20; AISBL; OTUD7C; TNFA1P2; TNFAIP3; Tnfip3

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UniProt ID: (Human) P21580, (Mouse) Q60769

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Entrez Gene ID: (Human) 7128, (Rat) 683206, (Mouse) 21929

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Function(s)
protease binding DNA binding ubiquitin-protein transferase activity thiol-dependent ubiquitin-specific protease activity protein binding zinc ion binding ligase activity kinase binding ubiquitin binding protein self-association K63-linked polyubiquitin binding catalytic activity peptidase activity cysteine-type peptidase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity hydrolase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity cysteine protease
Process(es)
B-1 B cell homeostasis response to molecule of bacterial origin regulation of germinal center formation negative regulation of chronic inflammatory response apoptotic process inflammatory response regulation of tumor necrosis factor-mediated signaling pathway protein deubiquitination negative regulation of protein ubiquitination negative regulation of NF-kappaB transcription factor activity negative regulation of type I interferon production response to muramyl dipeptide negative regulation of interleukin-1 beta production negative regulation of interleukin-2 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production negative regulation of toll-like receptor 2 signaling pathway negative regulation of toll-like receptor 3 signaling pathway negative regulation of toll-like receptor 4 signaling pathway negative regulation of toll-like receptor 5 signaling pathway protein K11-linked deubiquitination negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of protein catabolic process negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of bone resorption negative regulation of innate immune response negative regulation of smooth muscle cell proliferation regulation of defense response to virus by host regulation of inflammatory response negative regulation of inflammatory response negative regulation of B cell activation protein oligomerization regulation of vascular wound healing cellular response to hydrogen peroxide nucleotide-binding oligomerization domain containing signaling pathway negative regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway negative regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway protein K63-linked deubiquitination protein K48-linked ubiquitination protein K48-linked deubiquitination cellular response to lipopolysaccharide protein deubiquitination involved in ubiquitin-dependent protein catabolic process tolerance induction to lipopolysaccharide establishment of protein localization to vacuole negative regulation of osteoclast proliferation negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of cellular protein catabolic process positive regulation of hepatocyte proliferation negative regulation of CD40 signaling pathway negative regulation of endothelial cell apoptotic process negative regulation of cell death marginal zone B cell differentiation negative regulation of granuloma formation regulation of immunoglobulin production proteolysis metabolic process response to wounding negative regulation of autophagy negative regulation of heterotypic cell-cell adhesion regulation of innate immune response
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