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TRADD Antibody (PA5-106869) in ICC/IF
Immunocytochemistry analysis of TRADD in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with TRADD polyclonal antibody (Product # PA5-106869) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106869
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TRADD(Accession Q15628), corresponding to amino acid residues M1-A31.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854533
Product Specific Information
Antibody detects endogenous levels of total TRADD.
Target Information
TRADD (Tumor necrosis factor receptor type 1-associated DEATH domain protein) is an important mediator of FAS and TNFR1 associated apoptotic signaling. The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12. TRADD acts by interacting with TRIP12 and leading to the disruption of the interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A. TRADD acts as an adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interation with FADD. Overexpression of TRADD leads to two major TNF-induced responses - apoptosis and activation of NF-kappa-B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hs89862; MGC11078; TNF receptor 1 associated signal transducer; TNFR1-associated DEATH domain protein; TNFRSF1A-associated via death domain; tradd; tumor necrosis factor receptor type 1 associated death domain protein; Tumor necrosis factor receptor type 1-associated DEATH domain protein; tumor necrosis factor receptor-1-associated protein
Gene Aliases: 9130005N23Rik; AA930854; Hs.89862; TRADD
UniProt ID: (Human) Q15628, (Mouse) Q3U0V2
Entrez Gene ID: (Human) 8717, (Rat) 246756, (Mouse) 71609
apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
protein heterooligomerization
positive regulation of hair follicle development
extrinsic apoptotic signaling pathway
activation of cysteine-type endopeptidase activity involved in apoptotic process
signal transduction
I-kappaB kinase/NF-kappaB signaling
extrinsic apoptotic signaling pathway via death domain receptors
regulation of tumor necrosis factor-mediated signaling pathway
regulation of necrotic cell death
tumor necrosis factor-mediated signaling pathway
positive regulation of apoptotic process
positive regulation of NF-kappaB transcription factor activity
death-inducing signaling complex assembly
activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of apoptotic process
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