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Invitrogen
TRADD Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
TRADD Antibody (PA5-34807) in IHC (P)
Immunohistochemistry (Paraffin) analysis of TRADD was performed in paraffin-embedded human endometrial carcinoma tissue using TRADD Polyclonal Antibody (Product # PA5-34807) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Product Details
PA5-34807
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 85 and 312 of TRADD (Uniprot ID#Q15628)
Conjugate
Form
Liquid
Concentration
0.85 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552159
Product Specific Information
Recommended positive controls: MCF-7, MDA-MB-231.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TRADD (Tumor necrosis factor receptor type 1-associated DEATH domain protein) is an important mediator of FAS and TNFR1 associated apoptotic signaling. The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12. TRADD acts by interacting with TRIP12 and leading to the disruption of the interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A. TRADD acts as an adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interation with FADD. Overexpression of TRADD leads to two major TNF-induced responses - apoptosis and activation of NF-kappa-B.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hs89862; MGC11078; TNFR1-associated DEATH domain protein; TNFRSF1A-associated via death domain; tumor necrosis factor receptor type 1 associated death domain protein; Tumor necrosis factor receptor type 1-associated DEATH domain protein; tumor necrosis factor receptor-1-associated protein
Gene Aliases: Hs.89862; TRADD
UniProt ID: (Human) Q15628
Entrez Gene ID: (Human) 8717
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
signal transduction
I-kappaB kinase/NF-kappaB signaling
extrinsic apoptotic signaling pathway via death domain receptors
regulation of tumor necrosis factor-mediated signaling pathway
regulation of necrotic cell death
tumor necrosis factor-mediated signaling pathway
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of NF-kappaB transcription factor activity
protein heterooligomerization
positive regulation of hair follicle development
death-inducing signaling complex assembly
extrinsic apoptotic signaling pathway
activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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