Proteintech

TRAF3 Polyclonal Antibody

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TRAF3 Antibody (18099-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 18099-1-AP) TRAF3 antibody at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

TRAF3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

18099-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 17 publications 17 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Human, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TRAF3 Fusion Protein Ag12185 (6-351 aa encoded by BC075087)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.42 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KMDSPGALQ TNPPLKLHTD RSAGTPVFVP EQGGYKEKFV KTVEDKYKCE KCHLVLCSPK QTECGHRFCE SCMAALLSSS SPKCTACQES IVKDKVFKDN CCKREILALQ IYCRNESRGC AEQLMLGHLL VHLKNDCHFE ELPCVRPDCK EKVLRKDLRD HVEKACKYRE ATCSHCKSQV PMIALQKHED TDCPCVVVSC PHKCSVQTLL RSELSAHLSE CVNAPSTCSF KRYGCVFQGT NQQIKAHEAS SAVQHVNLLK EWSNSLEKKV SLLQNESVEK NKSIQSLHNQ ICSFEIEIER QKEMLRNNES KILHLQRVID SQAEKLKELD KEIRPFRQNW EEADSMK (6-351 aa encoded by BC075087)

Target Information

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e. g. RANK, CD30, CD40, etc. ) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF3, originally named CRAF1, interacts directly with the CD40 cytoplasmic tail through a region of similarity to the tumor necrosis factor-alpha (TNF-alpha) receptor-associated factors. TRAF3 binds only a single site, which contains the sequence PEQET, whereas TRAF1 and TRAF2 are capable of binding to either the PEQET site or an additional downstream domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion.

Signal transduction and targeted therapy

Zheng W,Zhou Z,Rui Y,Ye R,Xia F,Guo F,Liu X,Su J,Lou M,Yu XF

Fri Feb 24 00:00:00 EST 2023

TDRD3 is an antiviral restriction factor that promotes IFN signaling with G3BP1.

PLoS pathogens

Deater M,Tamhankar M,Lloyd RE

Sat Jan 01 00:00:00 EST 2022

Synthesis and anti-inflammatory activity of paeonol derivatives with etherized aryl urea by regulating TLR4/MyD88 signaling pathway in RAW264.7 cell.

Bioorganic chemistry

Gong X,He Y,Yang D,Yang S,Li J,Zhao H,Chen Q,Ren Q,Zhang B

Sat Oct 01 00:00:00 EDT 2022

Expression of miRNA-29 in Pancreatic β Cells Promotes Inflammation and Diabetes via TRAF3.

Cell reports

Sun Y,Zhou Y,Shi Y,Zhang Y,Liu K,Liang R,Sun P,Chang X,Tang W,Zhang Y,Li J,Wang S,Zhu Y,Han X

Tue Jan 05 00:00:00 EST 2021

TNF receptor-associated factor 3 restrains B-cell receptor signaling in normal and malignant B cells.

The Journal of biological chemistry

Whillock AL,Ybarra TK,Bishop GA

Fri Aug 27 00:00:00 EDT 2021

Hypothermic oxygenated perfusion inhibits HECTD3-mediated TRAF3 polyubiquitination to alleviate DCD liver ischemia-reperfusion injury.

Cell death & disease

Zhou W,Zhong Z,Lin D,Liu Z,Zhang Q,Xia H,Peng S,Liu A,Lu Z,Wang Y,Ye S,Ye Q

Wed Feb 24 00:00:00 EST 2021

Evolutionary history and functional characterization of Lj-TICAM-a and Lj-TICAM-b formed via lineage-specific tandem duplication in lamprey (Lampetra japonica).

Genomics

Geng M,Hua Y,Liu Y,Quan J,Hu X,Su P,Li Y,Liu X,Li Q,Zhu T

Thu Jul 01 00:00:00 EDT 2021

The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.

Nucleic acids research

Zong X,Xiao X,Shen B,Jiang Q,Wang H,Lu Z,Wang F,Jin M,Min J,Wang F,Wang Y

Fri Jun 04 00:00:00 EDT 2021

Enterovirus D68 Protease 2A^pro Targets TRAF3 To Subvert Host Innate Immune Responses.

Journal of virology

Kang J,Pang Z,Zhou Z,Li X,Liu S,Cheng J,Liu P,Tan W,Wang Z,Wang T

Wed Jan 13 00:00:00 EST 2021

TRIM24 facilitates antiviral immunity through mediating K63-linked TRAF3 ubiquitination.

The Journal of experimental medicine

Zhu Q,Yu T,Gan S,Wang Y,Pei Y,Zhao Q,Pei S,Hao S,Yuan J,Xu J,Hou F,Wu X,Peng C,Wu P,Qin J,Xiao Y

Mon Jul 06 00:00:00 EDT 2020

The Nucleoprotein of H7N9 Influenza Virus Positively Regulates TRAF3-Mediated Innate Signaling and Attenuates Viral Virulence in Mice.

Journal of virology

Wei Y,Zeng Y,Zhang X,Xu S,Wang Z,Du Y,Zhang B,Lei CQ,Zhu Q

Mon Nov 23 00:00:00 EST 2020

TRAF3 Is Required for NF-κB Pathway Activation Mediated by HTLV Tax Proteins.

Frontiers in microbiology

Fochi S,Bergamo E,Serena M,Mutascio S,Journo C,Mahieux R,Ciminale V,Bertazzoni U,Zipeto D,Romanelli MG

Thu Oct 01 00:00:00 EDT 2020

Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators.

The EMBO journal

Robeson AC,Lindblom KR,Wojton J,Kornbluth S,Matsuura K

Fri Jul 13 00:00:00 EDT 2018

Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators.

The EMBO journal

Robeson AC,Lindblom KR,Wojton J,Kornbluth S,Matsuura K

Fri Jul 13 00:00:00 EDT 2018

Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.

Cancer letters

Wang B,Zhao H,Zhao L,Zhang Y,Wan Q,Shen Y,Bu X,Wan M,Shen C

Wed Nov 01 00:00:00 EDT 2017

Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.

Cancer letters

Wang B,Zhao H,Zhao L,Zhang Y,Wan Q,Shen Y,Bu X,Wan M,Shen C

Wed Nov 01 00:00:00 EDT 2017

The C-Terminal Effector Domain of Non-Structural Protein 1 of Influenza A Virus Blocks IFN-β Production by Targeting TNF Receptor-Associated Factor 3.

Frontiers in immunology

Qian W,Wei X,Guo K,Li Y,Lin X,Zou Z,Zhou H,Jin M

Wed Nov 20 00:00:00 EST 2019

The C-Terminal Effector Domain of Non-Structural Protein 1 of Influenza A Virus Blocks IFN-β Production by Targeting TNF Receptor-Associated Factor 3.

Frontiers in immunology

Qian W,Wei X,Guo K,Li Y,Lin X,Zou Z,Zhou H,Jin M

Wed Nov 20 00:00:00 EST 2019

Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.

Mediators of inflammation

Jiang Z,Song F,Li Y,Xue D,Deng G,Li M,Liu X,Wang Y

Tue Mar 27 00:00:00 EDT 2018

Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.

Mediators of inflammation

Jiang Z,Song F,Li Y,Xue D,Deng G,Li M,Liu X,Wang Y

Tue Mar 27 00:00:00 EDT 2018

Protective Effects of Dioscin against Lipopolysaccharide-Induced Acute Lung Injury through Inhibition of Oxidative Stress and Inflammation.

Frontiers in pharmacology

Yao H,Sun Y,Song S,Qi Y,Tao X,Xu L,Yin L,Han X,Xu Y,Li H,Sun H,Peng J

Wed Sep 30 00:00:00 EDT 2020

Protective Effects of Dioscin against Lipopolysaccharide-Induced Acute Lung Injury through Inhibition of Oxidative Stress and Inflammation.

Frontiers in pharmacology

Yao H,Sun Y,Song S,Qi Y,Tao X,Xu L,Yin L,Han X,Xu Y,Li H,Sun H,Peng J

Wed Sep 30 00:00:00 EDT 2020

Ring finger protein 166 potentiates RNA virus-induced interferon-β production via enhancing the ubiquitination of TRAF3 and TRAF6.

Scientific reports

Chen HW,Yang YK,Xu H,Yang WW,Zhai ZH,Chen DY

Mon Oct 12 00:00:00 EDT 2015

Ring finger protein 166 potentiates RNA virus-induced interferon-β production via enhancing the ubiquitination of TRAF3 and TRAF6.

Scientific reports

Chen HW,Yang YK,Xu H,Yang WW,Zhai ZH,Chen DY

Mon Oct 12 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD40 associated protein 1; CD40 binding protein; CD40 receptor associated factor 1; CD40 receptor-associated factor 1; CD40-binding protein; CD40BP; CRAF1; LAP1; LMP1-associated protein 1; RING-type E3 ubiquitin transferase TRAF3; TNF receptor-associated factor 3

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Gene Aliases: CAP-1; CAP1; CD40bp; CRAF1; IIAE5; LAP1; TRAF3; TRAFAMN

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UniProt ID: (Human) Q13114

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Entrez Gene ID: (Human) 7187

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Function(s)

ubiquitin-protein transferase activity signal transducer activity tumor necrosis factor receptor binding protein binding zinc ion binding ligase activity protein kinase binding protein phosphatase binding ubiquitin protein ligase binding thioesterase binding scaffold/adaptor protein

Process(es)

regulation of cytokine production toll-like receptor signaling pathway apoptotic process signal transduction Toll signaling pathway protein ubiquitination regulation of proteolysis negative regulation of NF-kappaB transcription factor activity negative regulation of type I interferon production regulation of interferon-beta production tumor necrosis factor-mediated signaling pathway TRIF-dependent toll-like receptor signaling pathway regulation of apoptotic process innate immune response regulation of defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TRAF3 Polyclonal Antibody

TRAF3 Antibody (18099-1-AP) in WB

Product Details

18099-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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