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Invitrogen
TRF2 Monoclonal Antibody (4A794.15)
FIGURE: 1 / 8
TRF2 Antibody (MA1-41001) in IHC (P)
Immunohistochemical analysis of TRF2 in Transitional cell carcinoma, urinary bladder. Samples were incubated in TRF2 monoclonal antibody (Product # MA1-41001) using a dilution of 4 µg/mL followed by a peroxidase-conjugate and DAB chromogen. Note specific nuclear staining. Tumor/normal adjacent tissue array slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue section... View More
Product Details
MA1-41001
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Deer,
Human,
Marsupial,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
4A794.15
Immunogen
Full-length TRF2 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2201326
Product Specific Information
The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature.
This antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M).
TRF2 forms missing the B and M domains are often referred to as mutant TRF2.Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain.
Suggested positive control: HL-60, antigen standard for TERF2 (transient overexpression lysate).
Target Information
TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length. TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes. TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini. The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells. Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Telomeric DNA-binding protein; telomeric repeat binding protein 2; Telomeric repeat-binding factor 2; TTAGGG repeat-binding factor 2
Gene Aliases: TERF2; TRBF2; TRF2
UniProt ID: (Human) Q15554, (Mouse) O35144
Entrez Gene ID: (Human) 7014, (Rat) 361403, (Mouse) 21750
telomere maintenance
in utero embryonic development
positive regulation of gene expression
negative regulation of gene expression
obsolete telomere maintenance via telomere shortening
telomere capping
telomeric loop formation
protection from non-homologous end joining at telomere
negative regulation of telomere maintenance
positive regulation of telomere maintenance
positive regulation of nitric-oxide synthase activity
protein localization to chromosome, telomeric region
negative regulation of beta-galactosidase activity
negative regulation of cellular senescence
cell cycle
regulation of telomere maintenance
telomere maintenance via telomerase
negative regulation of telomere maintenance via recombination
regulation of telomere maintenance via telomerase
negative regulation of telomere maintenance via semi-conservative replication
cellular senescence
negative regulation of telomere single strand break repair
negative regulation of telomere capping
negative regulation of t-circle formation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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