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Talin Antibody (MA5-47740) in WB
Western blot of Talin in rat PC12 cells stimulated with Calyculin A (100 nM) for 30 minutes (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse Talin monoclonal antibody (Product # MA5-47740) (lanes 1 & 2) or rabbit polyclonal antibody anti-Talin (Ser425) (lanes 3 & 4).
Product Details
MA5-47740
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
8D4
Immunogen
Clone 8D4 was generated from purified chicken gizzard talin. The antibody recognizes an epitope within the rod domain of talin from many species, including human, rat, mouse, chicken, and frog.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942721
Product Specific Information
The antibody detects a 240 kDa* protein corresponding to the molecular mass of Talin on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells.
Target Information
Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: talin 2; Talin-1; Talin-2
Gene Aliases: 5730421P04Rik; AI507121; AI787438; AL118320; ILWEQ; KIAA0320; KIAA1027; mKIAA0320; RGD1565416; talin-1; talin-2; TLN; TLN1; TLN2
UniProt ID: (Chicken) P54939, (Human) Q9Y490, (Human) Q9Y4G6, (Mouse) P26039, (Mouse) Q71LX4
Entrez Gene ID: (Chicken) 395194, (Human) 7094, (Chicken) 415374, (Human) 83660, (Rat) 313494, (Mouse) 21894, (Mouse) 70549, (Rat) 315776
structural constituent of cytoskeleton
protein binding
clathrin binding
clathrin light chain binding
phosphatidylinositol binding
clathrin adaptor activity
phosphatidylinositol-3,4-bisphosphate binding
actin filament binding
phosphatidylinositol-3,5-bisphosphate binding
integrin binding
vinculin binding
LIM domain binding
cadherin binding involved in cell-cell adhesion
actin binding
structural molecule activity
molecular_function
insulin receptor binding
protein complex binding
non-motor actin binding protein
endocytosis
actin filament organization
cell adhesion
positive regulation of receptor-mediated endocytosis
clathrin coat assembly
platelet degranulation
movement of cell or subcellular component
muscle contraction
cytoskeletal anchoring at plasma membrane
cell-cell junction assembly
cell-substrate junction assembly
viral process
cortical actin cytoskeleton organization
IRE1-mediated unfolded protein response
platelet aggregation
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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