Invitrogen

Tau (Cleaved Asp421, Asp422) Monoclonal Antibody (TauC3)

15 Published Figures

15 References

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)
Published Figures (15)

Tau (Cleaved Asp421, Asp422) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

Tau (Cleaved Asp421, Asp422) Antibody (AHB0061) in ICC/IF

Immunofluorescent analysis of Tau (Cleavage site 421/422) Antibody (TauC3) was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Tau (Cleavage site 421/422) Antibody (TauC3) (Product # AHB0061) at 1µg/mL in 1% BSA... View More

Tau (Cleaved Asp421, Asp422) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Tau (Cleaved Asp421, Asp422) Antibody in Immunohistochemistry (IHC)

Tau (Cleaved Asp421, Asp422) Antibody in Western Blot (WB)

Tau (Cleaved Asp421, Asp422) Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

Product Details

AHB0061

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1 µg/mL

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

TauC3

Immunogen

KLH-CSSTGSIDMVD, corresponding to the C terminus of tau truncated at Asp421.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536237

Product Specific Information

This antibody recognizes tau when truncated at Asp421. Several caspases, including caspase-3, caspase-7, and caspase-8, cleave tau at this site, yielding the truncated form of the protein. This antibody does not react with full-length tau, or with tau truncated at Glu391 or Ala429. This antibody provides a tool for studying neuronal cell apoptosis.

Recommended Positive Control: Tau and caspase-3 cleaved tau.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclindependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Activity-dependent tau cleavage by caspase-3 promotes neuronal dysfunction and synaptotoxicity.

iScience

Opland CK,Bryan MR,Harris B,McGillion-Moore J,Tian X,Chen Y,Itano MS,Diering GH,Meeker RB,Cohen TJ

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Immunoprecipitation

Fri Jun 16 00:00:00 EDT 2023

Activity-dependent tau cleavage by caspase-3 promotes neuronal dysfunction and synaptotoxicity.

iScience

Opland CK,Bryan MR,Harris B,McGillion-Moore J,Tian X,Chen Y,Itano MS,Diering GH,Meeker RB,Cohen TJ

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Immunoprecipitation

Fri Jun 16 00:00:00 EDT 2023

Direct and Indirect Effects of Filamin A on Tau Pathology in Neuronal Cells.

Molecular neurobiology

Levert S,Pilliod J,Aumont É,Armanville S,Tremblay C,Calon F,Leclerc N

AHB0061 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to indicate that in Tauopathies, FLNA could contribute to Tau pathology by acting on Tau and annexin A2.

Wed Feb 01 00:00:00 EST 2023

Unconventional secretion of tau by VAMP8 impacts its intra- and extracellular cleavage.

Frontiers in cell and developmental biology

Pilliod J,Gélinas-Faucher M,Leclerc N

AHB0061 was used in Western Blotting to indicate that VAMP8 overexpression affects the intra- and extracellular cleavage pattern of Tau.

Wed Nov 02 00:00:00 EDT 2022

Targeting MicroRNA-485-3p Blocks Alzheimer's Disease Progression.

International journal of molecular sciences

Koh HS,Lee S,Lee HJ,Min JW,Iwatsubo T,Teunissen CE,Cho HJ,Ryu JH

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Western Blot

Sat Dec 04 00:00:00 EST 2021

Early-life exposure to high-fat diet influences brain health in aging mice.

Aging cell

Di Meco A,Praticò D

AHB0061 was used in Western Blotting to investigate the effect that maternal high-fat diet during gestation has on brain health of the offspring later in life.

Sun Dec 01 00:00:00 EST 2019

Early-life exposure to high-fat diet influences brain health in aging mice.

Aging cell

Di Meco A,Praticò D

AHB0061 was used in Western Blotting to investigate the effect that maternal high-fat diet during gestation has on brain health of the offspring later in life.

Sun Dec 01 00:00:00 EST 2019

Early-life exposure to high-fat diet influences brain health in aging mice.

Aging cell

Di Meco A,Praticò D

AHB0061 was used in Western Blotting to investigate the effect that maternal high-fat diet during gestation has on brain health of the offspring later in life.

Sun Dec 01 00:00:00 EST 2019

A unique tau conformation generated by an acetylation-mimic substitution modulates P301S-dependent tau pathology and hyperphosphorylation.

The Journal of biological chemistry

Ajit D,Trzeciakiewicz H,Tseng JH,Wander CM,Chen Y,Ajit A,King DP,Cohen TJ

AHB0061 was used in Western Blotting to indicate that a complex tau PTM code likely regulates tau pathogenesis, highlighting the potential utility of manipulating and detoxifying tau strains through site-specific tau-targeting approaches.

Fri Nov 08 00:00:00 EST 2019

Relevance of Phosphorylation and Truncation of Tau to the Etiopathogenesis of Alzheimer's Disease.

Frontiers in aging neuroscience

Zhou Y,Shi J,Chu D,Hu W,Guan Z,Gong CX,Iqbal K,Liu F

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Western Blot

Sat Apr 09 00:00:00 EDT 2022

Modifications of tau protein after cerebral ischemia and reperfusion in rats are similar to those occurring in Alzheimer's disease - Hyperphosphorylation and cleavage of 4- and 3-repeat tau.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Fujii H,Takahashi T,Mukai T,Tanaka S,Hosomi N,Maruyama H,Sakai N,Matsumoto M

AHB0061 was used in Western Blotting to study the pathological association of tau dynamics in both cerebral ischemia and Alzheimer's disease.

Sat Jul 01 00:00:00 EDT 2017

Pimozide reduces toxic forms of tau in TauC3 mice via 5' adenosine monophosphate-activated protein kinase-mediated autophagy.

Journal of neurochemistry

Kim YD,Jeong EI,Nah J,Yoo SM,Lee WJ,Kim Y,Moon S,Hong SH,Jung YK

AHB0061 was used in Western Blotting to study the mechanism by which pimozide can rescue tau pathology in an Alzheimer's disease model.

Fri Sep 01 00:00:00 EDT 2017

The identification of raft-derived tau-associated vesicles that are incorporated into immature tangles and paired helical filaments.

Neuropathology and applied neurobiology

Nishikawa T,Takahashi T,Nakamori M,Hosomi N,Maruyama H,Miyazaki Y,Izumi Y,Matsumoto M

AHB0061 was used in immunohistochemistry - paraffin section to study the relationship between neurofibrillary tangles and raft domains

Thu Dec 01 00:00:00 EST 2016

Subacute Changes in Cleavage Processing of Amyloid Precursor Protein and Tau following Penetrating Traumatic Brain Injury.

PloS one

Cartagena CM,Mountney A,Hwang H,Swiercz A,Rammelkamp Z,Boutte AM,Shear DA,Tortella FC,Schmid KE

AHB0061 was used in western blot to ascertain the effects of severe penetrating traumatic brain injury on APP and tau cleavage processing

Wed Jul 26 00:00:00 EDT 2017

Subacute Changes in Cleavage Processing of Amyloid Precursor Protein and Tau following Penetrating Traumatic Brain Injury.

PloS one

Cartagena CM,Mountney A,Hwang H,Swiercz A,Rammelkamp Z,Boutte AM,Shear DA,Tortella FC,Schmid KE

AHB0061 was used in western blot to ascertain the effects of severe penetrating traumatic brain injury on APP and tau cleavage processing

Wed Jul 26 00:00:00 EDT 2017

Carboxy terminus heat shock protein 70 interacting protein reduces tau-associated degenerative changes.

Journal of Alzheimer's disease : JAD

Saidi LJ,Polydoro M,Kay KR,Sanchez L,Mandelkow EM,Hyman BT,Spires-Jones TL

AHB0061 was used in western blot to test if CHIP ameliorates the pathological changes associated with tau aggregation.

Mon Oct 26 00:00:00 EDT 2015

Pericyte loss influences Alzheimer-like neurodegeneration in mice.

Nature communications

Sagare AP,Bell RD,Zhao Z,Ma Q,Winkler EA,Ramanathan A,Zlokovic BV

Published figure using Tau (Cleaved Asp421, Asp422) monoclonal antibody (Product # AHB0061) in Immunohistochemistry

Tue Jul 08 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: G protein beta1/gamma2 subunit-interacting factor 1; map tau; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Paired helical filament-tau; PHF-tau; protein phosphatase 1, regulatory subunit 103; Tau microtubule-associated protein

View more View less

Gene Aliases: AI413597; AW045860; DDPAC; FTDP-17; MAPT; MAPTL; MSTD; Mtapt; MTBT1; MTBT2; PPND; PPP1R103; pTau; RNPTAU; TAU

View more View less

UniProt ID: (Human) P10636, (Mouse) P10637

View more View less

Entrez Gene ID: (Human) 4137, (Rat) 29477, (Mouse) 17762

View more View less

Function(s)

structural constituent of cytoskeleton protein binding microtubule binding SH3 domain binding enzyme binding protein kinase binding protein complex binding apolipoprotein binding protein phosphatase 2A binding lipoprotein particle binding protein domain specific binding tubulin binding

Process(es)

microtubule cytoskeleton organization neuron migration brain development female pregnancy response to nutrient adult walking behavior axo-dendritic transport intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance regulation of autophagy regulation of microtubule polymerization positive regulation of microtubule polymerization negative regulation of intracellular transport positive regulation of axon extension mitochondrion transport along microtubule axon extension generation of neurons regulation of microtubule-based movement apoptotic process microtubule-based process axonogenesis neuron differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Tau (Cleaved Asp421, Asp422) Monoclonal Antibody (TauC3)

Tau (Cleaved Asp421, Asp422) Antibody (AHB0061) in ICC/IF

Product Details

AHB0061

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!