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UBC3B Antibody (PA5-114787) in ICC/IF
Immunocytochemistry analysis of UBC3B in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114787) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-114787
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human UBE2R2(Accession Q712K3), corresponding to amino acid residues D208-S238.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899423
Product Specific Information
Antibody detects endogenous levels of total UBE2R2.
Target Information
Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2 ubiquitin-conjugating enzyme R2; E2-CDC34B; Ubiquitin carrier protein R2; ubiquitin conjugating enzyme E2R 2; Ubiquitin-conjugating enzyme E2 R2; Ubiquitin-conjugating enzyme E2-CDC34B; ubiquitin-conjugating enzyme E2R 2; ubiquitin-conjugating enzyme UBC3B; Ubiquitin-protein ligase R2
Gene Aliases: 1200003M11Rik; CDC34B; E2-CDC34B; UBC3B; UBE2R2
UniProt ID: (Human) Q712K3, (Mouse) Q6ZWZ2
Entrez Gene ID: (Human) 54926, (Mouse) 67615
Performance Guarantee
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