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UBE1L Antibody (MA5-50749) in IHC (P)
Immunohistochemistry of UBE1L in paraffin-embedded mouse cerebellum tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with UBE1L recombinant monoclonal antibody (Product # MA5-50749) with a dilution of 1:1,000 for 1 hour at r... View More
Product Details
MA5-50749
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE50-55
Immunogen
Recombinant protein within Human Ube1L aa 1-100/1012.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3092430
Product Specific Information
Positive control: THP-1 cell lysates, rat kidney tissue, human testis tissue, mouse cerebellum tissue.
Predicted band size: 112 kDa
Subcellular Location: Cytoplasm, cytosol, nucleoplasm, nucleus.
Target Information
UBE1L (ubiquitin-like modifier-activating enzyme 7) activates ubiquitin by first adenylating with ATP at its C-terminal glycine residue and then linking that residue to the side chain of a cysteine residue in E1. This activation results in an ubiquitin-E1 thioester and free AMP. UBE1L also catalyzes the ISGylation of influenza A virus NS1 protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: D8; MGC12713; UBA1, ubiquitin-activating enzyme E1 homolog B; UBA7, ubiquitin-activating enzyme E1; Ubiquitin-activating enzyme 7; Ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-related protein; ubiquitin-activating enzyme-2; ubiquitin-like modifier activating enzyme 7; Ubiquitin-like modifier-activating enzyme 7
Gene Aliases: 1300004C08Rik; D8; UBA1B; UBA7; UBE1L; UBE2
UniProt ID: (Human) P41226, (Mouse) Q9DBK7
Entrez Gene ID: (Human) 7318, (Mouse) 74153, (Rat) 301000
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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