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UBE2B Antibody (PA5-120986) in WB
Western blot analysis of UBE2B in extracts of various cell lines. Samples were incubated with UBE2B Polyclonal antibody (Product # PA5-120986) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-120986
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2B (NP_003328.1).
Conjugate
Form
Liquid
Concentration
1.61 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914558
Product Specific Information
Positive test controls include: MCF7, SW480, Jurkat, NIH/3T3, Mouse heart, Mouse brain, Mouse skeletal muscle, Rat heart, Rat brain. The target is usually found in the following locations: Cell membrane, Nucleus.
Immunogen sequence: MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN DS
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2 protein; E2 ubiquitin-conjugating enzyme B; E2(14k); E214K; HR6B; RAD6 homolog B; Ubiquitin carrier protein B; ubiquitin conjugating enzyme; ubiquitin conjugating enzyme E2B; Ubiquitin-conjugating enzyme E2 B; Ubiquitin-conjugating enzyme E2(14k); Ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog); ubiquitin-conjugating enzyme E2B (RAD6 homolog, S. cerevisiae); ubiquitin-conjugating enzyme E2B, RAD6 homolog; ubiquitin-conjugating enzyme E2B, RAD6 homology; Ubiquitin-protein ligase B
Gene Aliases: 2610301N02Rik; E2-14k; E2-17kDa; HHR6B; HR6B; mHR6B; RAD6B; UBC2; UBCH1; UBE2B
UniProt ID: (Human) P63146, (Rat) P63149, (Mouse) P63147
Entrez Gene ID: (Human) 7320, (Rat) 81816, (Mouse) 22210
protein polyubiquitination
response to hypoxia
in utero embryonic development
DNA repair
postreplication repair
maintenance of chromatin silencing
ubiquitin-dependent protein catabolic process
protein monoubiquitination
cellular response to DNA damage stimulus
spermatogenesis
sperm axoneme assembly
response to UV
positive regulation of reciprocal meiotic recombination
protein ubiquitination
histone ubiquitination
regulation of histone modification
cellular response to insulin stimulus
negative regulation of histone phosphorylation
histone H2A ubiquitination
response to drug
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of cAMP-mediated signaling
protein stabilization
chiasma assembly
protein autoubiquitination
canonical Wnt signaling pathway
histone lysine demethylation
synaptonemal complex organization
protein K63-linked ubiquitination
protein K48-linked ubiquitination
protein K11-linked ubiquitination
DNA damage response, detection of DNA damage
meiotic telomere clustering
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