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UCP2 Antibody (ANT-128-200UL) in ICC/IF
Expression of Mitochondrial uncoupling protein 2 (UCP2) in mouse C2C12 cells. Immunocytochemical staining of mouse muscle myoblast (C2C12) fixed, permeabilized cells. Intracellular staining of cells with Anti-UCP2 Antibody (#ANT-128), (green), (1:50-1:200) followed by goat Anti-rabbit-AlexaFluor-488 secondary Antibody . A. Magnitude 1x40 (1:50). B-C. Magnitude 1x100 (1:200). DAPI is used as the counterstain (blue).
Product Details
ANT-128-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)GRRYQSTVEAYK, corresponding to amino acid residues 153-164 of rat mitochondrial uncoupling protein 2 (UCP2), Intracellular
Conjugate
Form
Lyophilized
Concentration
0.85 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP2 gene is expressed in many tissues, with the greatest expression in skeletal muscle. UCP2 is thought to play a role in non shivering thermogenesis, obesity and diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dicarboxylate carrier SLC25A8; Dicarboxylate carrier UCP2; Mitochondrial uncoupling protein 2; Solute carrier family 25 member 8; UCP 2; UCPH; uncoupling protein; uncoupling protein 2 (mitochondrial, proton carrier); Uncoupling protein 2, mitochondrial
Gene Aliases: BMIQ4; SLC25A8; UCP2; UCPH
UniProt ID: (Human) P55851, (Rat) P56500, (Mouse) P70406
Entrez Gene ID: (Human) 7351, (Rat) 54315, (Mouse) 22228
response to superoxide
response to hypoxia
translation
transport
mitochondrial transport
female pregnancy
aging
positive regulation of cell death
proton transport
cellular response to insulin stimulus
cellular response to amino acid starvation
negative regulation of apoptotic process
regulation of mitochondrial membrane potential
negative regulation of insulin secretion involved in cellular response to glucose stimulus
response to fatty acid
cellular response to glucose stimulus
liver regeneration
response to glucose
cellular response to hormone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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