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VAMP A/B Antibody (OSV00014W-100UL) in IHC (Free)
IHC on free floating sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for OCT embedding. Tissues were cut at 30 um thickness. Primary antibody: dilution 1:2000, incubated overnight.
Product Details
OSV00014W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from aa region 50-100 of human VAMPB conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
The peptide is homologous in many species including human, rat, mouse, zebra fish, bovine, xenopus and chicken.
Specificity of this antibody: VAMP A and B.
Target Information
VAPA/VAPB may play a role in vesicle trafficking. Present in the plasma membrane and in intracellular vesicles. Interacts with VAMP1 and VAMP2. Subunit: Homodimer, and heterodimer with VAPA. Subcellular Location: Cell membrane; Single-pass type IV membrane protein. Intracytoplasmic membrane; Single-pass type IV membrane protein. Tissue Specificity: Ubiquitous. Isoform 1 predominates. Disease: Defects in VAPB are the cause of amyotrophic lateral sclerosis type 8 (ALS8). ALS8 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. Defects in VAPB are a cause of proximal adult autosomal dominant spinal muscular atrophy; also called late-onset spinal muscular atrophy Finkel type. Spinal muscular atrophies are neurodegenerative disorders characterized by degeneration of lower motor neurons, leading to progressive paralysis muscular atrophy. This form is a late- adult-onset form of the disease (after age 20 years). The patients show a benign course, most of them remaining ambulatory 10 to 40 years after clinical onset.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 33 kDa VAMP-associated protein; synaptobrevin-associated protein of 33kDa; VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa; VAMP (vesicle-associated membrane protein)-associated protein B and C; VAMP-A; VAMP-associated 33 kDa protein; VAMP-associated protein 33b; VAMP-associated protein A; VAMP-associated protein B; VAMP-B; VAMP-B/VAMP-C; VAP-33; VAP-A; VAP-B; vesicle-associated membrane protein, associated protein a; vesicle-associated membrane protein, associated protein B and C; Vesicle-associated membrane protein-associated protein A; Vesicle-associated membrane protein-associated protein B; Vesicle-associated membrane protein-associated protein B/C
Gene Aliases: 33kDa; AI225786; AI840687; AI848259; ALS8; D2Abb2e; hVAP-33; R75548; UNQ484/PRO983; VAMP-B; Vamp33b; VAP-33; VAP-A; VAP-B; VAP33; VAP33b; VAPA; VAPB
UniProt ID: (Human) Q9P0L0, (Human) O95292, (Rat) Q9Z270, (Mouse) Q9WV55, (Rat) Q9Z269
Entrez Gene ID: (Human) 9218, (Human) 9217, (Rat) 58857, (Mouse) 30960, (Rat) 60431, (Mouse) 56491
signal transducer activity
protein binding
microtubule binding
protein domain specific binding
FFAT motif binding
protein heterodimerization activity
cadherin binding involved in cell-cell adhesion
enzyme binding
protein homodimerization activity
beta-tubulin binding
molecular_function
membrane traffic protein
membrane trafficking regulatory protein
ER to Golgi vesicle-mediated transport
endoplasmic reticulum organization
signal transduction
cell death
sphingolipid biosynthetic process
neuron projection development
protein folding in endoplasmic reticulum
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation by host of viral release from host cell
negative regulation by host of viral genome replication
positive regulation by host of viral genome replication
membrane fusion
protein localization to endoplasmic reticulum
COPII-coated vesicle budding
cell-cell adhesion
cellular calcium ion homeostasis
activation of signaling protein activity involved in unfolded protein response
modulation by virus of host morphology or physiology
endoplasmic reticulum unfolded protein response
positive regulation of viral genome replication
biological_process
negative regulation by host of viral release from host cell
negative regulation by virus of viral protein levels in host cell
response to unfolded protein
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