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VAMP8 Antibody (PA5-89528) in WB
Western blot analysis of VAMP8 in extracts of various cell lines. Samples were incubated with VAMP8 Polyclonal antibody (Product # PA5-89528) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-89528
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-75 of human VAMP8 (NP_0037522)
Conjugate
Form
Liquid
Concentration
0.56 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805630
Product Specific Information
Immunogen sequence: MEEASEGGGN DRVRNLQSEV EGVKNIMTQN VERILARGEN LEHLRNKTED LEATSEHFKT TSQKVARKFW WKNVK; Positive Samples: HeLa, NIH/3T3, PC-12, Mouse kidney, Mouse liver, Rat lung; Cellular Location: Cell membrane, Early endosome membrane, Late endosome membrane, Lysosome membrane, Single-pass type IV membrane protein
Target Information
Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EDB; Endobrevin; vamp; VAMP-8; Vesicle-associated membrane protein 8; vesicle-associated membrane protein 8 (endobrevin)
Gene Aliases: AU041171; EDB; endobrevin; VAMP-8; VAMP8
UniProt ID: (Human) Q9BV40, (Mouse) O70404, (Rat) Q9WUF4
Entrez Gene ID: (Human) 8673, (Mouse) 22320, (Rat) 83730
protein complex assembly
transport
exocytosis
vesicle fusion
autophagy
protein transport
vesicle-mediated transport
regulation of endocytosis
SNARE complex assembly
viral entry into host cell
membrane fusion
mucus secretion
autophagosome maturation
regulation of protein localization to plasma membrane
negative regulation of secretion by cell
positive regulation of histamine secretion by mast cell
endocytosis
eosinophil degranulation
neutrophil degranulation
post-Golgi vesicle-mediated transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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