Proteintech

VAPA Polyclonal Antibody

VAPA Antibody in Immunocytochemistry (ICC/IF)

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VAPA Antibody (15275-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VAPA antibody (Product # 15275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

VAPA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VAPA Antibody in Immunoprecipitation (IP)

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Product Details

15275-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

VAPA Fusion Protein Ag7393 (1-221 aa encoded by BC002992)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.42 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MASASGAMA KHEQILVLDP PTDLKFKGPF TDVVTTNLKL RNPSDRKVCF KVKTTAPRRY CVRPNSGIID PGSTVTVSVM LQPFDYDPNE KSKHKFMVQT IFAPPNTSDM EAVWKEAKPD ELMDSKLRCV FEMPNENDKL NDMEPSKAVP LNASKQDGPM PKPHSVSLND TETRKLMEEC KRLQGEMMKL SEENRHLRDE GLRLRKVAHS DKPGSTSTAS FR (1-221 aa encoded by BC002992)

Target Information

The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Profiling of purified autophagic vesicle degradome in the maturing and aging brain.

Neuron

Kallergi E,Siva Sankar D,Matera A,Kolaxi A,Paolicelli RC,Dengjel J,Nikoletopoulou V

Wed Aug 02 00:00:00 EDT 2023

Elucidation of OSW-1-Induced Stress Responses in Neuro2a Cells.

International journal of molecular sciences

Oh-Hashi K,Nakamura H,Ogawa H,Hirata Y,Sakurai K

Fri Mar 17 00:00:00 EDT 2023

An obligate intracellular bacterial pathogen forms a direct, interkingdom membrane contact site.

bioRxiv : the preprint server for biology

Acevedo-Sánchez Y,Woida PJ,Kraemer S,Lamason RL

Tue Jun 06 00:00:00 EDT 2023

Host cell membrane proteins located near SARS-CoV-2 spike protein attachment sites are identified using proximity labeling and proteomic analysis.

The Journal of biological chemistry

Kotani N,Nakano T,Kuwahara R

Tue Nov 01 00:00:00 EDT 2022

Host cell membrane proteins located near SARS-CoV-2 spike protein attachment sites are identified using proximity labeling and proteomic analysis.

The Journal of biological chemistry

Kotani N,Nakano T,Kuwahara R

Tue Nov 01 00:00:00 EDT 2022

Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites.

Cell

Zheng Q,Chen Y,Chen D,Zhao H,Feng Y,Meng Q,Zhao Y,Zhang H

Thu Oct 27 00:00:00 EDT 2022

Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites.

Cell

Zheng Q,Chen Y,Chen D,Zhao H,Feng Y,Meng Q,Zhao Y,Zhang H

Thu Oct 27 00:00:00 EDT 2022

Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.

The Journal of cell biology

Culver JA,Mariappan M

Mon May 03 00:00:00 EDT 2021

Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.

EMBO reports

Spits M,Heesterbeek IT,Voortman LM,Akkermans JJ,Wijdeven RH,Cabukusta B,Neefjes J

Wed Mar 03 00:00:00 EST 2021

Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.

The Journal of cell biology

Nthiga TM,Shrestha BK,Bruun JA,Larsen KB,Lamark T,Johansen T

Mon Jun 07 00:00:00 EDT 2021

Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.

The Journal of cell biology

Nthiga TM,Shrestha BK,Bruun JA,Larsen KB,Lamark T,Johansen T

Mon Jun 07 00:00:00 EDT 2021

The ER-embedded UBE2J1/RNF26 ubiquitylation complex exerts spatiotemporal control over the endolysosomal pathway.

Cell reports

Cremer T,Jongsma MLM,Trulsson F,Vertegaal ACO,Neefjes J,Berlin I

Tue Jan 19 00:00:00 EST 2021

Extracellular calcium alters calcium-sensing receptor network integrating intracellular calcium-signaling and related key pathway.

Scientific reports

Gorkhali R,Tian L,Dong B,Bagchi P,Deng X,Pawar S,Duong D,Fang N,Seyfried N,Yang J

Mon Oct 18 00:00:00 EDT 2021

Extracellular calcium alters calcium-sensing receptor network integrating intracellular calcium-signaling and related key pathway.

Scientific reports

Gorkhali R,Tian L,Dong B,Bagchi P,Deng X,Pawar S,Duong D,Fang N,Seyfried N,Yang J

Mon Oct 18 00:00:00 EDT 2021

Extracellular calcium alters calcium-sensing receptor network integrating intracellular calcium-signaling and related key pathway.

Scientific reports

Gorkhali R,Tian L,Dong B,Bagchi P,Deng X,Pawar S,Duong D,Fang N,Seyfried N,Yang J

Mon Oct 18 00:00:00 EDT 2021

Extracellular calcium alters calcium-sensing receptor network integrating intracellular calcium-signaling and related key pathway.

Scientific reports

Gorkhali R,Tian L,Dong B,Bagchi P,Deng X,Pawar S,Duong D,Fang N,Seyfried N,Yang J

Mon Oct 18 00:00:00 EDT 2021

Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.

The Journal of cell biology

Liu N,Zhao H,Zhao YG,Hu J,Zhang H

Mon Jul 05 00:00:00 EDT 2021

CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.

The EMBO journal

Nthiga TM,Kumar Shrestha B,Sjøttem E,Bruun JA,Bowitz Larsen K,Bhujabal Z,Lamark T,Johansen T

Mon Aug 03 00:00:00 EDT 2020

Upregulation of Rubicon promotes autosis during myocardial ischemia/reperfusion injury.

The Journal of clinical investigation

Nah J,Zhai P,Huang CY,Fernández ÁF,Mareedu S,Levine B,Sadoshima J

Mon Jun 01 00:00:00 EDT 2020

Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.

Scientific reports

Yamanaka T,Nishiyama R,Shimogori T,Nukina N

Wed May 06 00:00:00 EDT 2020

A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complex.

The EMBO journal

Forrester A,De Leonibus C,Grumati P,Fasana E,Piemontese M,Staiano L,Fregno I,Raimondi A,Marazza A,Bruno G,Iavazzo M,Intartaglia D,Seczynska M,van Anken E,Conte I,De Matteis MA,Dikic I,Molinari M,Settembre C

Tue Jan 15 00:00:00 EST 2019

A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complex.

The EMBO journal

Tue Jan 15 00:00:00 EST 2019

A trap mutant reveals the physiological client spectrum of TRC40.

Journal of cell science

Coy-Vergara J,Rivera-Monroy J,Urlaub H,Lenz C,Schwappach B

Mon Jul 01 00:00:00 EDT 2019

MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.

Molecular cell

Freyre CAC,Rauher PC,Ejsing CS,Klemm RW

Thu Dec 05 00:00:00 EST 2019

The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.

Current biology : CB