Invitrogen

VAPB Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 Published Figures

3 References

View all (30) VAPB antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

VAPB Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 13

VAPB Antibody (PA5-53023) in IHC

Immunohistochemical staining of VAPB in human small intestine using a VAPB Polyclonal Antibody (Product # PA5-53023) shows strong cytoplasmic positivity in glandular cells.

VAPB Antibody in Immunoprecipitation (IP)

Product Image Gallery

VAPB Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of VAPB was observed specifically in Caco-2 cells and in HEK293 cells using anti-VAPB Polyclonal Antibody (Product # PA5-53023) in western blot. The relative expression levels of VAPB within each cell line is shown using RNA-Seq. {RE}

Product Details

PA5-53023

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

1:500-1:1,000

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Non-human primate, Rabbit

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human VAPB. Recombinant protein control fragment (Product #RP-90173).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2649391

Product Specific Information

Immunogen sequence: VWKEAKPEDL MDSKLRCVFE LPAENDKPHD VEINKIISTT ASKTETPIVS KSLSSSLDDT EVKKVMEECK RLQGEVQRLR EENKQFKEED GLRMRKTVQS NSPISALAPT GK

Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 79%.

Target Information

Vesicle-associated membrane protein-associated protein B/C is a protein that in humans is encoded by the VAPB gene. The VAPB gene is found on the 20th human chromosome. The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Osthole interacts with an ER-mitochondria axis and facilitates tumor suppression in ovarian cancer.

Journal of cellular physiology

Bae H,Lee JY,Song J,Song G,Lim W

PA5-53023 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate the natural derivative osthole as a promising anticancer compound against ovarian cancer and evaluated its ability to suppress and abrogate tumor progression.

Mon Feb 01 00:00:00 EST 2021

Osthole interacts with an ER-mitochondria axis and facilitates tumor suppression in ovarian cancer.

Journal of cellular physiology

Bae H,Lee JY,Song J,Song G,Lim W

Mon Feb 01 00:00:00 EST 2021

Role of Vesicle-Associated Membrane Protein-Associated Proteins (VAP) A and VAPB in Nuclear Egress of the Alphaherpesvirus Pseudorabies Virus.

Viruses

Dorsch AD,Hölper JE,Franzke K,Zaeck LM,Mettenleiter TC,Klupp BG

PA5-53023 was used in Western Blotting to mutate genes encoding VAPB and VAPA by CRISPR/Cas9-based genome editing in our standard rabbit kidney cells (RK13), either individually or in combination.

Thu Jun 10 00:00:00 EDT 2021

Role of Vesicle-Associated Membrane Protein-Associated Proteins (VAP) A and VAPB in Nuclear Egress of the Alphaherpesvirus Pseudorabies Virus.

Viruses

Dorsch AD,Hölper JE,Franzke K,Zaeck LM,Mettenleiter TC,Klupp BG

PA5-53023 was used in Western Blotting to mutate genes encoding VAPB and VAPA by CRISPR/Cas9-based genome editing in our standard rabbit kidney cells (RK13), either individually or in combination.

Thu Jun 10 00:00:00 EDT 2021

Role of Vesicle-Associated Membrane Protein-Associated Proteins (VAP) A and VAPB in Nuclear Egress of the Alphaherpesvirus Pseudorabies Virus.

Viruses

Dorsch AD,Hölper JE,Franzke K,Zaeck LM,Mettenleiter TC,Klupp BG

PA5-53023 was used in Western Blotting to mutate genes encoding VAPB and VAPA by CRISPR/Cas9-based genome editing in our standard rabbit kidney cells (RK13), either individually or in combination.

Thu Jun 10 00:00:00 EDT 2021

Stigmasterol Causes Ovarian Cancer Cell Apoptosis by Inducing Endoplasmic Reticulum and Mitochondrial Dysfunction.

Pharmaceutics

Bae H,Song G,Lim W

PA5-53023 was used in Western Blotting to investigate the effects of stigmasterol on proapoptotic signals, mitochondrial function, reactive oxygen species production, and the cytosolic and mitochondrial calcium levels in human ovarian cancer cells, to understand the mechanisms underlying the effects of stigmasterol on ovarian cancer cells.

Thu May 28 00:00:00 EDT 2020

Stigmasterol Causes Ovarian Cancer Cell Apoptosis by Inducing Endoplasmic Reticulum and Mitochondrial Dysfunction.

Pharmaceutics

Bae H,Song G,Lim W

Thu May 28 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: ALS 8; ALS8VAMP-B/VAMP-C; vamp; VAMP (vesicle-associated membrane protein)-associated protein B and C; Vamp 33b; VAMP B; VAMP B VAMP C; VAMP B/VAMP C; VAMP C; VAMP-associated 33 kDa protein; VAMP-associated protein 33b; VAMP-associated protein B; VAMP-B; VAMP-B/VAMP-C; VAMP-C; VAMPB; VAMPB/VAMPC; VAMPC; VAP 33b; VAP B; VAP B/VAP C; VAP C; VAP-B; VAP-B/VAP-C; VAP-C; VAPB/VAPC; VAPC; vesicle-associated membrane protein, associated protein B and C; Vesicle-associated membrane protein-associated protein B; Vesicle-associated membrane protein-associated protein B/C

View more View less

Gene Aliases: AI225786; AI840687; AI848259; ALS8; D2Abb2e; R75548; UNQ484/PRO983; VAMP-B; Vamp33b; VAP-B; VAP33b; VAPB

View more View less

UniProt ID: (Human) O95292, (Rat) Q9Z269

View more View less

Entrez Gene ID: (Human) 9217, (Rat) 60431, (Mouse) 56491

View more View less

Function(s)

protein binding microtubule binding enzyme binding FFAT motif binding protein homodimerization activity protein heterodimerization activity beta-tubulin binding cadherin binding involved in cell-cell adhesion molecular_function membrane traffic protein membrane trafficking regulatory protein

Process(es)

cellular calcium ion homeostasis ER to Golgi vesicle-mediated transport activation of signaling protein activity involved in unfolded protein response endoplasmic reticulum organization modulation by virus of host morphology or physiology sphingolipid biosynthetic process endoplasmic reticulum unfolded protein response positive regulation of viral genome replication COPII-coated vesicle budding cell-cell adhesion biological_process negative regulation by host of viral release from host cell positive regulation by host of viral release from host cell negative regulation by host of viral genome replication positive regulation by host of viral genome replication negative regulation by virus of viral protein levels in host cell response to unfolded protein

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

VAPB Polyclonal Antibody

VAPB Antibody (PA5-53023) in IHC

Product Details

PA5-53023

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!