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VARS Antibody (PA5-30251) in IHC (P)
VARS Polyclonal Antibody detects Valyl tRNA synthetase protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VARS Polyclonal Antibody (Product # PA5-30251) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-30251
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 46 and 270 of Human Valyl tRNA synthetase
Conjugate
Form
Liquid
Concentration
0.96 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547725
Product Specific Information
Recommended positive controls: A549, HeLa, HepG2, HCT116.
Predicted reactivity: Mouse (84%), Rat (86%), Pig (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. This protein belongs to the class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 6.1.1.9; Protein G7a; valine tRNA ligase 1, cytoplasmic; Valine--tRNA ligase; ValRS; valyl trna synthetase; Valyl-tRNA synthetase; valyl-tRNA synthetase 2
Gene Aliases: G7A; VARS; VARS1; VARS2
UniProt ID: (Human) B4DZ61
Entrez Gene ID: (Human) 7407
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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