Invitrogen

VEGF Receptor 2 Polyclonal Antibody

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Datasheet

Protocols

Tech Support

Antibody Testing Data (2)
Published Figures (5)

VEGF Receptor 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

VEGF Receptor 2 Antibody (PA5-16487) in IHC (P)

Formalin-fixed, paraffin-embedded human angiosarcoma stained with Flk-1/ VEGFR2 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

VEGF Receptor 2 Antibody in Western Blot (WB)

VEGF Receptor 2 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-16487

Applications

Tested Dilution

Publications

Western Blot (WB)

5-10 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat, Rhesus monkey

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from C-terminus of the precursor form of the mouse Flk-1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10978670

Product Specific Information

PA5-16487 targets Flk-1 in IHC (P) and WB applications and shows reactivity with mouse, Rat, and Human samples.

The PA5-16487 immunogen is a synthetic peptide from C-terminus of the precursor form of the mouse Flk-1.

Target Information

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeted Nanoparticles with High Heating Efficiency for the Treatment of Endometriosis with Systemically Delivered Magnetic Hyperthermia.

Small (Weinheim an der Bergstrasse, Germany)

Park Y,Demessie AA,Luo A,Taratula OR,Moses AS,Do P,Campos L,Jahangiri Y,Wyatt CR,Albarqi HA,Farsad K,Slayden OD,Taratula O

PA5-16487 was used in Immunohistochemistry (Frozen) to describe nanoparticles that enable systemically delivered magnetic hyperthermia for endometriosis treatment.

Wed Jun 01 00:00:00 EDT 2022

In vitro angiogenesis inhibition with selective compounds targeting the key glycolytic enzyme PFKFB3.

Pharmacological research

Abdali A,Baci D,Damiani I,Belloni F,De Dominicis C,Gelmi ML,Corsini A,Bellosta S

PA5-16487 was used in Western Blot to demonstrate that the anti-glycolytic PA compounds may affect several steps involved in angiogenesis.

Tue Jun 01 00:00:00 EDT 2021

SARS-CoV-2 spike protein co-opts VEGF-A/neuropilin-1 receptor signaling to induce analgesia.

Pain

Moutal A,Martin LF,Boinon L,Gomez K,Ran D,Zhou Y,Stratton HJ,Cai S,Luo S,Gonzalez KB,Perez-Miller S,Patwardhan A,Ibrahim MM,Khanna R

PA5-16487 was used in Western Blot to show that preventing VEGF-A/NRP-1 signaling was antiallodynic in a neuropathic pain model.

Fri Jan 01 00:00:00 EST 2021

Retinal Protection from LED-Backlit Screen Lights by Short Wavelength Absorption Filters.

Cells

Sanchez-Ramos C,Bonnin-Arias C,Blázquez-Sánchez V,Aguirre-Vilacoro V,Cobo T,García-Suarez O,Perez-Carrasco MJ,Alvarez-Peregrina C,Vega JA

Published figure using VEGF Receptor 2 polyclonal antibody (Product # PA5-16487) in Immunohistochemistry

Fri Nov 19 00:00:00 EST 2021

Hypoxia and proangiogenic proteins in human ameloblastoma.

Scientific reports

de Mendonça RP,Balbinot KM,Martins BV,da Silva Kataoka MS,Mesquita RA,de Jesus Viana Pinheiro J,de Melo Alves Júnior S

PA5-16487 was used in Immunohistochemistry to investigate the expression of the HIF-1α, MMP-2, VEGF, and VEGFR-2 proteins among 24 ameloblastoma cases, 10 calcifying odontogenic cysts, and 9 dental follicles by immunohistochemistry.

Fri Oct 16 00:00:00 EDT 2020

Hypoxia and proangiogenic proteins in human ameloblastoma.

Scientific reports

de Mendonça RP,Balbinot KM,Martins BV,da Silva Kataoka MS,Mesquita RA,de Jesus Viana Pinheiro J,de Melo Alves Júnior S

Fri Oct 16 00:00:00 EDT 2020

SARS-CoV-2 Spike protein co-opts VEGF-A/Neuropilin-1 receptor signaling to induce analgesia.

bioRxiv : the preprint server for biology

Moutal A,Martin LF,Boinon L,Gomez K,Ran D,Zhou Y,Stratton HJ,Cai S,Luo S,Gonzalez KB,Perez-Miller S,Patwardhan A,Ibrahim MM,Khanna R

PA5-16487 was used in Western Blot to test if the SARS-CoV-2 Spike protein could block VEGF-A/NRP-1 signaling.

Mon Sep 14 00:00:00 EDT 2020

A phase II study of tivozanib in patients with metastatic and nonresectable soft-tissue sarcomas.

Annals of oncology : official journal of the European Society for Medical Oncology

Agulnik M,Costa RLB,Milhem M,Rademaker AW,Prunder BC,Daniels D,Rhodes BT,Humphreys C,Abbinanti S,Nye L,Cehic R,Polish A,Vintilescu C,McFarland T,Skubitz K,Robinson S,Okuno S,Van Tine BA

PA516487 was used in immunohistochemistry to evaluate the use of Tivozanib to treat patients with soft tissue sarcomas

Sun Jan 01 00:00:00 EST 2017

Myoferlin expression in non-small cell lung cancer: Prognostic role and correlation with VEGFR-2 expression.

Oncology letters

Song DH,Ko GH,Lee JH,Lee JS,Lee GW,Kim HC,Yang JW,Heo RW,Roh GS,Han SY,Kim DC

PA5-16487 was used in immunohistochemistry - paraffin section and western blot to characterize the prognostic role of myoferlin expression in non-small cell lung cancer in correlation with VEGFR-2 expression

Mon Feb 01 00:00:00 EST 2016

Myoferlin expression in non-small cell lung cancer: Prognostic role and correlation with VEGFR-2 expression.

Oncology letters

Song DH,Ko GH,Lee JH,Lee JS,Lee GW,Kim HC,Yang JW,Heo RW,Roh GS,Han SY,Kim DC

Mon Feb 01 00:00:00 EST 2016

Expression profiling and significance of VEGF-A, VEGFR2, VEGFR3 and related proteins in endometrial carcinoma.

Cytokine

Wang J,Taylor A,Showeil R,Trivedi P,Horimoto Y,Bagwan I,Ewington L,Lam EW,El-Bahrawy MA

PA5-16487 was used in immunohistochemistry to study the prognostic and therapeutic value of the immunohistochemical expression of VEGF-A, VEGFR2 and VEGFR3 in patients with endometrial carcinoma

Fri Aug 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; FLK1 kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGF receptor 2); FLK1 kinase insert domain receptor (VEGF receptor 2); KDR; kinase insert domain protein receptor; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

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Gene Aliases: 6130401C07; CD309; Flk-1; FLK1; KDR; Krd-1; Ly73; sVEGFR-2; VEGFR; VEGFR-2; VEGFR2

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UniProt ID: (Human) P35968, (Rat) O08775, (Mouse) P35918

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Entrez Gene ID: (Human) 3791, (Rat) 25589, (Mouse) 16542

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Function(s)

protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity integrin binding protein binding ATP binding growth factor binding Hsp90 protein binding vascular endothelial growth factor binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor

Process(es)

Performance Guarantee

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VEGF Receptor 2 Polyclonal Antibody

VEGF Receptor 2 Antibody (PA5-16487) in IHC (P)

Product Details

PA5-16487

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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