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VHLH Antibody (BS-7883R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VHLH Polyclonal Antibody, Unconjugated (bs-7883R) at 1:400 overnight at 4°C, DAB staining.
Product Details
BS-7883R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human VHLH/Von Hippel Lindau, amino acids 101-200.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: elongin binding protein; Protein G7; pVHL; VHL19; VHLp18(MEA); Von Hippel Lindau; von hippel-lindau; von Hippel-Lindau disease tumor suppressor; von Hippel-Lindau syndrome homolog; von Hippel-Lindau syndrome protein homolog; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
Gene Aliases: HRCA1; pVHL; RCA1; VHL; VHL1; Vhlh
UniProt ID: (Human) P40337, (Mouse) P40338, (Rat) Q64259
Entrez Gene ID: (Human) 7428, (Mouse) 22346, (Rat) 24874
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
regulation of transcription, DNA-templated
proteolysis
negative regulation of cell proliferation
protein ubiquitination
negative regulation of apoptotic process
positive regulation of cell differentiation
positive regulation of transcription, DNA-templated
protein stabilization
regulation of transcription from RNA polymerase II promoter in response to hypoxia
negative regulation of transcription from RNA polymerase II promoter in response to hypoxia
angiogenesis
response to hypoxia
type B pancreatic cell differentiation
pancreatic A cell differentiation
NLS-bearing protein import into nucleus
proteasomal protein catabolic process
protein transport
protein catabolic process
neuron differentiation
extracellular matrix organization
regulation of catecholamine metabolic process
blood vessel endothelial cell migration
negative regulation of endothelial cell differentiation
protein heterooligomerization
regulation of thymocyte apoptotic process
negative regulation of thymocyte apoptotic process
negative regulation of hypoxia-inducible factor-1alpha signaling pathway
regulation of apoptotic signaling pathway
response to ethanol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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