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FIGURE: 1 / 1
VPS29 Antibody (PA5-88981) in WB
Western blot analysis of VPS29 in extracts of various cell lines. Samples were incubated with VPS29 Polyclonal antibody (Product # PA5-88981) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88981
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human VPS29 (NP_4765281)
Conjugate
Form
Liquid
Concentration
2.76 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805269
Product Specific Information
Immunogen sequence: MAGHRLVLVL GDLHIPHRCN SLPAKFKKLL VPGKIQHILC TGNLCTKESY DYLKTLAGDV HIVRGDFDEN LNYPEQKVVT VGQFKIGLIH GHQVIPWGDM ASLALLQRQF DVDILISGHT HKFEAFEHEN KFYINPGSAT GAYNALETNI IPSFVLMDIQ ASTVVTYVYQ LIGDDVKVER IEYKKP; Positive Samples: HT-29, 293T; Cellular Location: Cytoplasm, Early endosome, Endosome membrane, Late endosome, Membrane, Peripheral membrane protein
Target Information
This gene belongs to a group of vacuolar protein sorting genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms, and usage of multiple polyadenylation sites have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp564F0223; FLJ20492; hVPS29; PEP11 homolog; retromer protein; vacuolar protein sorting 29 homolog; Vacuolar protein sorting-associated protein 29; vacuolar protein sorting-associated protein 29 {ECO:0000250|UniProtKB:Q9UBQ0}; vacuolar sorting protein 29; vacuolar sorting protein VPS29/PEP11; Vesicle protein sorting 29; vesicle protein sorting 29 {ECO:0000250|UniProtKB:Q9UBQ0}; VPS29 retromer complex component; vps29 {ECO:0000312|EMBL:AAI67055.1, ECO:0000312|RGD:1308332}; x 007 protein
Gene Aliases: 2010015D08Rik; AW049835; DC15; DC7; MDS007; PEP11; VPS29
UniProt ID: (Human) Q9UBQ0, (Rat) B2RZ78, (Mouse) Q9QZ88
Entrez Gene ID: (Human) 51699, (Rat) 288666, (Mouse) 56433
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