Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
VSIG1 Antibody (PA5-89497) in WB
Western blot analysis of VSIG1 in extracts of various cell lines. Samples were incubated with VSIG1 Polyclonal antibody (Product # PA5-89497) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89497
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-230 of human VSIG1 (NP_8724131)
Conjugate
Form
Liquid
Concentration
1.46 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805609
Product Specific Information
Immunogen sequence: ASREQLSIQW SFFHKKEMEP ISIYFSQGGQ AVAIGQFKDR ITGSNDPGNA SITISHMQPA DSGIYICDVN NPPDFLGQNQ GILNVSVLVK PSKPLCSVQG RPETGHTISL SCLSALGTPS PVYYWHKLEG RDIVPVKENF NPTTGILVIG NLTNFEQGYY QCTAINRLGN SSCEIDLTSS H; Positive Samples: BxPC3, OVCAR3, Mouse testis, Mouse stomach, Mouse pancreas; Cellular Location: Membrane, Single-pass type I membrane protein
Target Information
VSIG1 encodes a member of the junctional adhesion molecule (JAM) family. The encoded protein contains multiple glycosylation sites at the N-terminal region, and multiple phosphorylation sites and glutamic acid/proline (EP) repeats at the C-terminal region. The gene is expressed in normal stomach and testis, as well as in gastric, esophageal and ovarian cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Cell surface A33 antigen; Glycoprotein A34; MGC44287; V-set and immunoglobulin domain-containing protein 1
Gene Aliases: 1700062D20Rik; 4930405J24Rik; dJ889N15.1; GPA34; VSIG1
UniProt ID: (Human) Q86XK7, (Mouse) Q9D2J4, (Rat) Q4KLY3
Entrez Gene ID: (Human) 340547, (Mouse) 78789, (Rat) 315920
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support