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WNT2B Antibody (701856) in ICC/IF
Immunofluorescence was performed on methanol fixed MDA-MB-231 cells for detection of WNT2B/13 using Anti-WNT2B/13 Recombinant Rabbit Monoclonal Antibody (Product # 701856, 2 µg/mL), alpha-Tubulin Monoclonal Antibody (Product # 32-2500, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000), Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594conjugate (Product # A-11032, 1:400) respectively. Panel a) shows representative cells that were stained... View More
Product Details
701856
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
17H30L23
Immunogen
Peptide corresponding to Human WNT2B (aa 286-298)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2606215
Product Specific Information
This antibody is predicted to react with Monkey, Pig and Rabbit.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
WNT2B is a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. It may play a role in human development as well as human carcinogenesis. Two alternatively spliced transcript variants exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein Wnt-13; Protein Wnt-2b; wingless related MMTV integration site 2b; wingless-type MMTV integration site family member 2B; wingless-type MMTV integration site family, member 13; wingless-type MMTV integration site family, member 2B; wnt-13; XWNT2, Xenopus, homolog of
Gene Aliases: WNT13; WNT2B
UniProt ID: (Human) Q93097, (Mouse) O70283
Entrez Gene ID: (Human) 7482, (Mouse) 22414
chondrocyte differentiation
lens development in camera-type eye
male gonad development
cellular response to starvation
Wnt signaling pathway
forebrain regionalization
neuron differentiation
cell fate commitment
canonical Wnt signaling pathway
lung induction
mesenchymal-epithelial cell signaling
iris morphogenesis
cornea development in camera-type eye
hematopoietic stem cell proliferation
positive regulation of branching involved in ureteric bud morphogenesis
positive regulation of canonical Wnt signaling pathway
signal transduction
cell-cell signaling
multicellular organism development
organ morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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