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Product Details
H00007490-D01P
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
WT1 (AAH32861.1, 1 a.a. approximately 302 a.a) full-length human protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Product may be used with Western Blot (Transfected lysate).
The Isotype of this product is composed of an IgG Mixture.
Immunogen sequence: MEKGYSTVTF DGTPSYGHTP SHHAAQFPNH SFKHEDPMGQ QGSLGEQQYS VPPPVYGCHT PTDSCTGSQA LLLRTPYSSD NLYQMTSQLE CMTWNQMNLG ATLKGVAAGS SSSVKWTEGQ SNHSTGYESD NHTTPILCGA QYRMHTHGVF RGIQDVRRVP GVAPTLVRSA SETSEKRPFM CAYPGCNKRY FKLSHLQMHS RKHTGEKPYQ CDFKDCERRF SRSDQLKRHQ RRHTGVKPFQ CKTCQRKFSR SDHLKTHTRT HTGEKPFSCR WPSCQKKFAR SDELVRHHNM HQRNMTKLQL AL
Target Information
The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Wilms tumor protein; Wilms tumor protein isoform Ex4a(+); WT; WT33
Gene Aliases: AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT1; WT33
UniProt ID: (Human) P19544
Entrez Gene ID: (Human) 7490
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
RNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
transcription regulatory region DNA binding
C2H2 zinc finger domain binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
vasculogenesis
ureteric bud development
branching involved in ureteric bud morphogenesis
kidney development
regulation of organ formation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
germ cell development
thorax and anterior abdomen determination
heart development
sex determination
negative regulation of cell proliferation
RNA splicing
gonad development
male gonad development
tissue development
negative regulation of cell growth
adrenal gland development
male genitalia development
epithelial cell differentiation
glomerulus development
glomerular basement membrane development
adrenal cortex formation
camera-type eye development
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
mesenchymal to epithelial transition
positive regulation of heart growth
diaphragm development
cardiac muscle cell fate commitment
visceral serous pericardium development
cellular response to cAMP
cellular response to gonadotropin stimulus
metanephric mesenchyme development
glomerular visceral epithelial cell differentiation
posterior mesonephric tubule development
metanephric epithelium development
metanephric S-shaped body morphogenesis
negative regulation of metanephric glomerular mesangial cell proliferation
positive regulation of male gonad development
negative regulation of female gonad development
positive regulation of metanephric ureteric bud development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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