Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
XAF1 Antibody (PA1-41099) in IHC (P)
Immunohistochemical analysis of XAF1 in immersion fixed paraffin-embedded sections of human appendix. Samples were incubated in XAF1 polyclonal antibody (Product # PA1-41099) using a dilution of 1:100 for 1 hour at room temperature followed by the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized t... View More
Product Details
PA1-41099
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptides, KLH conjugated, corresponding to residues 201-218 and 301-317 of human XaF1.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2216764
Product Specific Information
Suggested positive control: antigen standard for XAF1 (transient overexpression lysate), human liver tissue or protein.
Target Information
The inhibitors of apoptosis (IAP) proteins prevent cell death both in vitro and in vivo by virtue of inhibition of distinct caspases. The X-linked Inhibitor of Apoptosis, XIAP, is a key member of the IAP family of proteins. The caspase inhibiting activity of XIAP is negatively regulated by at least two XIAP-interacting proteins, XIAP-associated factor 1 (XAF1) and Smac/DIABLO. XAF1 binds to XIAP and antagonizes caspase inhibitory activities of XIAP. Expression of XAF1 triggers a redistribution of XIAP from the cytosol to the nucleus. XAF1 is ubiquitously expressed in normal tissues, but is present at low or undetectable levels in many different cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BIRC4 binding protein; BIRC4-binding protein; F-box protein 39; novel TRAF-type zinc finger protein; XIAP-associated factor 1
Gene Aliases: BIRC4BP; Fbox39; HSXIAPAF1; XAF1; XIAPAF1
UniProt ID: (Human) Q6GPH4, (Mouse) Q5NBU8
Entrez Gene ID: (Human) 54739, (Mouse) 327959
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support