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YBX1 Antibody (711277) in ICC/IF
For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized for detection of endogenous YB1 using Anti-YB1 Recombinant Rabbit Polyclonal Antibody (Product # 711277, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of YB1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Pa... View More
Product Details
711277
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
10HCLC
Immunogen
Peptides corresponding to human YB1 (aa174-190, 251-270)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2663065
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Mouse, Rat
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BP8; CBF-A; CCAAT-binding; CCAAT-binding transcription factor I subunit A; DBPB; DNA binding protein B; DNA-binding protein B; EFI-A; Enhancer factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; MDR NF1; MGC104858; MGC110976; MGC117250; nuclease sensitive element binding protein 1; Nuclease-sensitive element-binding protein 1; Y box binding protein 1; Y-box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YB-1
Gene Aliases: 1700102N10Rik; BP-8; C79409; CBF-A; CSDA2; CSDB; DBPB; EF1A; EFI-A; MDR-NF1; Msy-1; MSY1; mYB-1a; NSEP-1; NSEP1; YB-1; YB1; YBX1
UniProt ID: (Human) P67809, (Mouse) P62960
Entrez Gene ID: (Human) 4904, (Mouse) 22608
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
double-stranded DNA binding
single-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
RNA binding
protein binding
poly(A) RNA binding
GTPase binding
p53 binding
nucleic acid binding
chromatin binding
mRNA binding
transcription factor binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
mRNA processing
positive regulation of cell proliferation
RNA splicing
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin receptor signaling pathway
negative regulation of striated muscle cell differentiation
positive regulation of cell division
CRD-mediated mRNA stabilization
mRNA splicing, via spliceosome
protein localization to cytoplasmic stress granule
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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