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Invitrogen
alanyl-tRNA Synthetase Polyclonal Antibody
FIGURE: 1 / 3
alanyl-tRNA Synthetase Antibody (PA5-29516) in IHC (P)
alanyl-tRNA Synthetase Polyclonal Antibody detects AARS protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Alanyl-tRNA Synthetase Polyclonal Antibody (Product # PA5-29516) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-29516
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 461 and 723 of Human alanyl-tRNA synthetase
Conjugate
Form
Liquid
Concentration
0.23 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546992
Product Specific Information
Recommended positive controls: A431, H1299, HeLa, HepG2, mouse liver.
Predicted reactivity: Mouse (95%), Rat (92%), Xenopus laevis (84%), Chicken (86%), Bovine (92%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E. coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AARS; alanine tRNA ligase 1, cytoplasmic; Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase; alanyl-tRNA synthetase, cytoplasmic; AlaRS; Protein lactyltransferase AARS1; Protein sticky; Renal carcinoma antigen NY-REN-42; Sti
Gene Aliases: AARS; AARS1; AI316495; C76919; CMT2N; EIEE29; sti
UniProt ID: (Human) P49588, (Mouse) Q8BGQ7
Entrez Gene ID: (Human) 16, (Mouse) 234734
tRNA binding
aminoacyl-tRNA editing activity
alanine-tRNA ligase activity
ATP binding
zinc ion binding
amino acid binding
nucleotide binding
nucleic acid binding
RNA binding
aminoacyl-tRNA ligase activity
protein binding
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
metal ion binding
aminoacyl-tRNA synthetase
hair follicle development
tRNA modification
tRNA aminoacylation for protein translation
alanyl-tRNA aminoacylation
regulation of translational fidelity
protein folding
tRNA processing
cerebellar Purkinje cell layer development
endoplasmic reticulum unfolded protein response
response to amino acid
negative regulation of neuron apoptotic process
neuromuscular process controlling balance
translation
cellular response to unfolded protein
tRNA aminoacylation
skin development
neuromuscular process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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